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Hct ion trap

Manufactured by Bruker
Sourced in Germany, United Kingdom

The HCT ion trap is a high-performance mass spectrometry instrument. It is designed to provide accurate and reliable mass analysis of a wide range of samples. The core function of the HCT ion trap is to capture, store, and detect ions for mass spectrometry analysis.

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3 protocols using hct ion trap

1

Quantifying PFOS in Exposure Tanks

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For quantification of PFOS in exposure tanks, 1 mL of water sample was collected from each fish tank shortly after exposure (day 0) and before renewing with fresh treatment water at day 5. Samples were stored at 4 °C, and PFOS were measured by combined liquid (Agilent 1200, American) mass spectrometry (Bruker Esquire HCT ion trap, Germany) (LC/MS) according to our previous methods44 (link)45 (link). Three independent replicates of each sample were prepared and analyzed. The concentrations of PFOS were calculated from standard curves and the average extraction efficiency of PFOS ranged from 75% to 80%.
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2

Elemental and Spectroscopic Analysis of Compounds

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Elemental analyses were performed by the microanalytical service of the Faculty of Chemistry of the University of Vienna on a Perkin–Elmer 2400 CHN Elemental Analyzer. UV/Vis spectra were recorded at 25 °C using a Perkin–Elmer Lambda 650 spectrometer equipped with an optical cell of 1 cm path-length in the wavelength range of 200 to 800 nm in combination with a Perkin–Elmer PTP-6 Peltier System. Electrospray ionization (ESI) mass spectrometry measurements were conducted on a Bruker HCT ion trap (Bruker Daltonics GmbH) by using methanol as a solvent. MIR spectra were recorded on a Perkin–Elmer 370 FTIR 2000 instrument using an ATR (attenuated total reflection) unit in the range of 4000–400 cm−1. Phosphorescence emission spectra were recorded with a Horiba FluoroMax-4 spectrofluorimeter and the data were processed using the FluorEssence v3.5 software package.
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3

Glucosinolate Extraction and Analysis Protocol

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Glucosinolates were extracted and analysed according to the protocol in Bell et al. (2015) (link) with the following alterations: Extracts were filtered with 0.22 μm Arcrodisc syringe filters with Supor membrane (hydrophilic polyethersulfone; VWR, Lutterworth, UK) after extraction. Analysis was performed using an Agilent 1200 Series LC system (Agilent, Stockport, UK) equipped with a variable wavelength detector (GSLs quantified at 229 nm), and coupled with a Bruker HCT ion trap (Bruker, Coventry, UK). A Gemini 3 μm C18 110 Å (150 × 4.6 mm) column was utilised (with Security Guard column, C18; 4 mm × 3 mm; Phenomenex, Macclesfield, UK), and separation was optimised for use with the Bell et al. (2015) (link) isocratic gradient, at a flow rate of 0.4 ml/min. A six point sinigrin hydrate calibration curve was prepared (r2 = 0.977, y = 7.763; Jin et al., 2009 (link)). Compounds were identified using literature ion data and characteristic ion fragments (Table 1). Quantification was performed using Bruker Daltonics HyStar software (Bruker) with relative response factors (Clarke, 2010 ).
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