Taqman probes

Manufactured by Eppendorf

TaqMan® probes are short DNA sequences labeled with a fluorescent reporter and a quencher dye. They are designed to specifically bind to a target DNA sequence during PCR amplification, allowing for real-time detection and quantification of the target gene.

Automatically generated - may contain errors

Lab products found in correlation

Realplex4 system, by Eppendorf (1 mentions) Quantitect cdna synthesis kit, by Qiagen (1 mentions) Mastercycler realplex4, by Eppendorf (1 mentions)

2 protocols using taqman probes

Analysis of Immune Gene Expression

Cited 3 times

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Total RNA was isolated using the TRIzol method. One microgram of RNA was reverse transcribed using the Quantitect cDNA synthesis kit. TRAF3IP2, cytokine, chemokine and adhesion molecule expression was analyzed by RT-qPCR using TaqMan® probes and Eppendorf Realplex⁴ system [11 (link), 12 (link), 14 (link)]. Data were shown as fold change (2^−ΔΔCt).

Valente A.J., Irimpen A.M., Siebenlist U, & Chandrasekar B. (2014). OxLDL induces endothelial dysfunction and death via TRAF3IP2. Inhibition by HDL3 and AMPK activators. Free radical biology & medicine, 70, 117-128.

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Quantitative RT-PCR for MMP2 and RECK

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Total RNA was isolated using the TRIzol method. One microgram of DNA-free total RNA was used for the first-strand cDNA synthesis using Quantitect cDNA Synthesis Kit (Qiagen, Germantown, MD). MMP2 and RECK mRNA levels were analyzed by RT-qPCR using TaqMan probes (Table S4) and MasterCycler RealPlex4 from Eppendorf. Glyceraldehyde-3-phosphate dehydrogenase served as an invariant control. Data are shown as fold change (2^−ΔΔC_t).

Mummidi S., Das N.A., Carpenter A.J., Yoshida T., Yariswamy M., Mostany R., Izadpanah R., Higashi Y., Sukhanov S., Noda M., Siebenlist U., Rector R.S, & Chandrasekar B. (2019). RECK suppresses interleukin-17/TRAF3IP2-mediated MMP-13 activation and human aortic smooth muscle cell migration and proliferation. Journal of cellular physiology, 234(12), 22242-22259.

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