Leaf phenotyping was performed as described in our previous study [24 (link)]. Briefly, the first completely uncurled leaf was defined as the first leaf. The fifth leaves of OE and CK plants were detached, fixed with FAA (formaldehyde: acetic acid: 96% alcohol: water; 10:5:50:35), cleared with chloral solution (200 g chloral hydrate, 20 g glycerol, and 50 mL dH2O), and surveyed using a confocal Zeiss LSM 510 AX70 microscope. The cell number in the lower epidermis was calculated by dividing the leaf area by the area of epidermal cells. At least six leaves were used for the leaf area measurements and more than 100 epidermal cells in each leaf were used for cell area measurements.
Lsm 510 ax70 microscope
Manufactured by Zeiss
The LSM 510 AX70 microscope is a high-performance confocal laser scanning microscope designed for advanced imaging applications. It offers high-resolution, multi-dimensional imaging capabilities for a wide range of samples.
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5 protocols using lsm 510 ax70 microscope
1
Leaf Epidermal Cell Phenotyping
2
Leaf Epidermal Cell Phenotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Leaf phenotyping was performed as described in our previous study [24] . Brie y, the rst completely uncurled leaf was de ned as the rst leaf. The fth leaves of OE and CK plants were detached, xed with FAA (formaldehyde: acetic acid: 96% alcohol: water; 10:5:50:35), cleared with chloral solution (200 g chloral hydrate, 20 g glycerol, and 50 mL dH 2 O), and surveyed using a confocal Zeiss LSM 510 AX70 microscope. The cell number in the lower epidermis was calculated by dividing the leaf area by the area of epidermal cells. At least six leaves were used for the leaf area measurements and more than 100 epidermal cells in each leaf were used for cell area measurements.
3
Leaf Epidermal Cell Phenotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Leaf phenotyping was performed as described in our previous study [24] . Brie y, the rst completely uncurled leaf was de ned as the rst leaf. The fth leaves of OE and CK plants were detached, xed with FAA (formaldehyde: acetic acid: 96% alcohol: water; 10:5:50:35), cleared with chloral solution (200 g chloral hydrate, 20 g glycerol, and 50 mL dH 2 O), and surveyed using a confocal Zeiss LSM 510 AX70 microscope. The cell number in the lower epidermis was calculated by dividing the leaf area by the area of epidermal cells. At least six leaves were used for the leaf area measurements and more than 100 epidermal cells in each leaf were used for cell area measurements.
4
Leaf Epidermal Cell Phenotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Leaf phenotyping was performed as described in our previous study [24] . Brie y, the rst completely uncurled leaf was de ned as the rst leaf. The fth leaves of OE and CK plants were detached, xed with FAA (formaldehyde: acetic acid: 96% alcohol: water; 10:5:50:35), cleared with chloral solution (200 g chloral hydrate, 20 g glycerol, and 50 mL dH 2 O), and surveyed using a confocal Zeiss LSM 510 AX70 microscope. The cell number in the lower epidermis was calculated by dividing the leaf area by the area of epidermal cells. At least six leaves were used for the leaf area measurements and more than 100 epidermal cells in each leaf were used for cell area measurements.
5
Leaf Epidermal Cell Phenotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Leaf phenotyping was performed as described in our previous study [24] . Brie y, the rst completely uncurled leaf was de ned as the rst leaf. The fth leaves of OE and CK plants were detached, xed with FAA (formaldehyde: acetic acid: 96% alcohol: water; 10:5:50:35), cleared with chloral solution (200 g chloral hydrate, 20 g glycerol, and 50 mL dH 2 O), and surveyed using a confocal Zeiss LSM 510 AX70 microscope. The cell number in the lower epidermis was calculated by dividing the leaf area by the area of epidermal cells. At least six leaves were used for the leaf area measurements and more than 100 epidermal cells in each leaf were used for cell area measurements.
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