The CCK-8 kit (cat: C0038, Beyotime, China) was used to assess the viability of HASMCs. In brief, the treated HASMCs (1 × 104 cells/well, 100 μL) were grown in a 96-well plate. At the indicated time, 10 µL of CCK-8 was incubated with the cells for 4 h at 37°C away from light. Then, the absorbance (450 nm) was detected using a Microplate Reader (PLUS 384, Molecular Devices, USA).
Plus 384
Manufactured by Molecular Devices
Sourced in United States
The PLUS 384 is a high-throughput microplate reader that can perform absorbance, fluorescence, and luminescence measurements in 384-well microplates. It features a compact design and automated operation for efficient data collection.
Automatically generated - may contain errors
11 protocols using plus 384
1
Assessing HASMC Viability with CCK-8
2
Cell Viability Assay with MTT
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Normal bone marrow cell line HS-5, AML cell lines (HL60, KG1, Kasumi-1, and NB4), as well as transfected HL60 and NB4 cells were collected and cell concentration was adjusted. Then, cells were cultured in 96-well plates for 24 or 48 h according to the instructions of MTT assay kit (C0009S; Beyotime). Next, 10 μL of MTT solution was added to the sample wells for further 4 h of incubation in the incubator. Thereafter, the cells were treated with 100 μL of Formazan solution and cultured in an incubator until Formazan was completely dissolved. Finally, the optical density values of sample wells were determined at a wavelength of 570 nm using a microplate reader (PLUS 384; Molecular Devices, California, USA).
3
Cell Viability Assessment via MTT Assay
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The cells (2 × 103 cells/well) were seeded into a 96-well plate. After 24 h, 48 h or 72 h of culture, cell viability was detected using MTT reagent (cat: C0009, Beyotime, China). After incubating the cells with MTT at 37°C for 4 h, formazan reagent (100 μl) was added into each well to dissolve the purple crystal. Then, the absorbance at 570 nm was measured under a microplate reader (PLUS 384; Molecular Devices, USA).
4
Hippocampal Neuron Viability Assay
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The viability of hippocampal neurons was measured by MTT assay. Briefly, the neurons (2,000 cells/well) were cultured in 96-well culture plates for 24 h. Then, MTT solution (5 mg/ml) was added into each well at 37°C for 4 h. Next, MTT was removed, and 150 µl DMSO were added into the wells to solubilize purple formazan crystals. Absorbance at 570 nm was then read using a microplate reader (PLUS 384, Molecular Devices, LLC).
5
Hypoxia Impacts Dermal Papilla Cell Proliferation
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Dermal papilla cells were seeded and cultured in 96‐well plates either in normoxia (20% O2) or hypoxia (5% O2) at an initial density of 4 × 103 cells per well. During the different time points at 6, 12, 24, 48 and 72 hours, 10 μL CCK‐8 reagent was added to each well and cells were incubated for 1.5 hours at 37°C. The absorbance was measured at a wavelength of 450 nm using a microplate reader (SpectraMax, Plus 384 Molecular Devices, Inc). For clone formation, transfected DPCs were counted and seeded into 6‐well plates (1000 cells per wall). Cells culture medium was replaced every 3 days. Cells colonies were counted after 14 days after fixation and dyeing with crystal violet.
6
Cell Viability Assay After UVB Irradiation
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Following UVB irradiation, PECs were incubated in 96-well plates at 100 µl/well with 5 replicate wells for each group for 24 h. Subsequently, 10 µl CCK8 was added to each well for analysis of cell viability according to the manufacturer's protocol (Shanghai Yeasen Biotech Co., Ltd.). Blank control wells were set and incubated at 37°C in the dark. The optical density (OD) value was read at a wavelength of 450 nm using a microplate reader Plus384 (Molecular Devices LLC) and the cell survival rate was calculated using the following formula: Cell viability (%)=(experimental group OD450/normal group OD450) ×100. The experiment was repeated three times.
7
LDH Cytotoxicity Assay in 96-well Plates
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The cells were cultured in 96-well plates, and the medium in each well was then collected and centrifuged at 1,000 × g, at 4°C for 10 min. The LDH in the supernatant was measured using a Cytotoxicity Detection kit (LDH, 11644793001). Subsequently, 100 µl reaction reagent were mixed with 100 µl supernatant and incubated for 30 min at room temperature. The absorbance was then read at 490 nm using a multimode reader (PLUS 384, Molecular Devices). The data were normalized to those of the cells in control group.
8
Neuronal Viability Assessment via MTT Assay
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The viability of neurons was measured by MTT assay. Briefly, the neurons (2,000 cells/well) were cultured in 96-well culture plates for 24 h. Then, MTT (5 mg/ml) was added into each well at 37°C for 4 h. After that, MTT was removed and 150 µl DMSO was added into the well to solubilize purple formazan. Then, absorbance at 570 nm was read using a microplate reader (PLUS 384; Molecular Devices, LLC). LDH cytotoxicity detection kit (cat. no. C0017; Beyotime Institute of Biotechnology) was used as previously described (22 (link)).
9
MTT-Based Cell Viability Assay
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Briefly, cells (4,000 cells/well) were seeded in a 96-well plate. Following culture for 48 h, the medium was removed and 10 µl MTT solution mixed with 110 µl fresh medium were added to each well. Following incubation at 37°C for 4 h, the medium was removed, and 150 µl DMSO was added to each well. The absorbance value at 570 nm was then detected using a microplate reader (PLUS 384, Molecular Devices, LLC).
10
Measuring Cell Viability with CCK-8
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Cell Counting Kit-8 (70-CCK801, MultiSciences, China) was used to determine the cell viability. Briefly, the GC cells (5 ×103 per) were grown into a 96-well plate after the transfection. Next, 10 µL CCK-8 solution was added into each well and held for 24h, 48h and 72h at 37°C, respectively. The absorbance value was detected at the wavelength of 490nm by a microplate reader (PLUS 384, Molecular Devices, USA).
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