HIOs were removed from Matrigel and washed 3-4 times in phosphate-buffered saline and subsequently incubated in TrypLE Express (Life Technologies, Camarillo, CA, USA) for 2-3 minutes until the organoids were completely disassociated to a single cell suspension. Single cells were then fixed and permeabilized using Flow Cytometry Fixation & Permeabilization Buffer Kit I (R&D Systems). Cells were then stained with the following fluorophore-conjugated antibodies: mouse anti-human EpCAM (# 324212; BioLegend, San Diego, CA, USA) and mouse anti-human vimentin (#562338; BD Pharmingen, San Diego, CA, USA). Cells were analyzed on a BD Fortessa Cell Analyzer (BD Biosciences) and flow cytometry analysis was carried out using FlowJo software (FlowJo, Ashland, OR, USA).
Mouse anti human vimentin
Manufactured by BD
Sourced in United States
Mouse anti-human vimentin is a primary antibody that recognizes the vimentin protein, which is a type III intermediate filament protein expressed in various cell types. This antibody can be used in immunohistochemistry, Western blotting, and other immunoassays to detect and study the expression of vimentin.
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Lab products found in correlation
2 protocols using mouse anti human vimentin
1
Dissociation and Profiling of HIO Cells
2
Protein Expression Analysis in AEII Cells
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Protein extraction from AEII at 21 days, and Western blotting was carried out as described previously (Cabrera-Benitez et al. 2012 (link)). The following antibodies were used: mouse anti-human E-cadherin antibody (BD Bioscience, 1:2000, San Jose, CA), goat anti-human pro-SP-B polyclonal antibody (Santa Cruz Biotechnology, 1:500), mouse anti-human vimentin (1:1000, BD), rabbit anti-human GAPDH (1:10000, BD), goat anti-mouse, donkey anti-goat, goat anti-rabbit-conjugated to horseradish peroxidase (1:5000, EarthOx).
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