Lipase from pseudomonas sp

Manufactured by Merck Group

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Lipase from Pseudomonas sp. is an enzyme that catalyzes the hydrolysis of lipids. It is extracted from the bacterium Pseudomonas sp. The core function of this enzyme is to break down fats and oils.

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Lipase (128 citations)

3 protocols using lipase from pseudomonas sp

Synthesis of Biopolymer Composites

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Lipase from Pseudomonas sp. (CAS No. 9004-02-8), succinic acid (SA; CAS No. 110-15-6), titanium(IV) isopropoxide (CAS No. 546-68-9), and zinc nitrate (CAS No. 7779-88-6) were obtained from Sigma-Aldrich Chemical Company, Saint Louis, MO, USA. Acrylic acid (AA; CAS No. 79-10-7), azobisisobutyronitrile (CAS No. 78-67-1), 1,4-butanediol (BD; CAS No. 2082-81-7), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC; CAS No. 1892-57-5), 1,6-diaminohexane (CAS No. 124-09-4), dimethylene terephthalate (DMT; CAS No. 120-61-6), and tetrabutyl titanate (CAS No. 5593-70-4) were purchased from Alfa Aesar Materials Company, Haverhill, MA, USA. Finally, 1,4-dioxane (CAS No. 123-91-1) was acquired from Macron Fine Chemicals, Radnor, PA, USA. All chemicals were utilized without further purification.

Lin S.H., Wang H.T., Wang J.M, & Wu T.M. (2020). Enzymatic Degradation of Acrylic Acid-Grafted Poly(butylene succinate-co-terephthalate) Nanocomposites Fabricated Using Heat Pressing and Freeze-Drying Techniques. Materials, 13(2), 376.

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Enzymatic Synthesis of Polyesters

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Lipase from Pseudomonas sp., dodecylamine (DDA), octadecylamine (ODA), zinc nitrate, and phenylphosphonic acid was acquired from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). 1,4-butanediol (BD), dimethylene carbonate (DMC), and dimethylene terephthalate (DMT) were obtained from Alfa Aesar Chemical Company (Haverhill, MA, USA). Sodium hydroxide was purchased from Fluka Chemical Company (Buchs, Switzerland). All chemicals were used without purification.

Tseng L.Y., Chen E.C., Wang J.M, & Wu T.M. (2020). Synthesis, Physical Properties and Enzymatic Degradation of Biodegradable Nanocomposites Fabricated Using Poly(Butylene Carbonate-Co-Terephthalate) and Organically Modified Layered Zinc Phenylphosphonate. Polymers, 12(9), 2149.

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Synthesis and Characterization of PLGA-PEG-PLGA Triblocks

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All water referred to in this article was deionised (Arium 611UV; Satorius, Germany; resistivity ¼ 18.2 MU. cm). Unstabilized tetrahydrofuran (THF) was obtained from Biosolve (Valkenswaard, The Netherlands).
Poly(D,L-lactide-co-glycolide)-block-poly(ethyleneglycol)-block-poly(D,L-lactide-co-glycolide) copolymer [PLGA-PEG-PLGA triblocks, number-average molecular weight, M n ¼ 6000-12000, PEG block M n ¼ 400, feed ratio lactide:glycolide 1:0.8], 4,4' bis(diethylamino) benzophenone (DEBP), ethylene glycol diacrylate, methyl methacrylate, 2,2-dimethoxy-2phenylacetophenone, butyl methacrylate, ethylene glycol dimethacrylate, Al 2 O 3, 1,4-butanediol, poly(ethylene glycol) methacrylate, tert-butanol, lipase from Pseudomonas Sp. (!1200 U/mg), NaCl, ethanolamine, trypsin from bovine pancreas, and proteinase K from Tritirachium album were obtained from Sigma Aldrich (Steinheim, Germany). Acetone was obtained from Klinipath (VWR, Duiven, The Netherlands). Polystyrene and poly(methyl methacrylate) standards for construction of the calibration curves were obtained from Polymer Laboratories (now Agilent Technologies, Church Stretton, Shropshire, UK). 1-propanol was obtained from Acros (Geel, Belgium) and 2-vinyl-4,4-dimethylazlactone was obtained from Pure Chemistry Scientific (Watertown, MA, United States of America).

Wouters B., Pirok B.W.J., Soulis D., Garmendia Perticarini R.C., Fokker S., van den Hurk R.S., Skolimowski M., Peters R.A.H, & Schoenmakers P.J. (2019). On-line microfluidic immobilized-enzyme reactors: A new tool for characterizing synthetic polymers. Analytica chimica acta, 1053.

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