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Anti hla dr apc cy7 clone l243

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Anti-HLA-DR APC-Cy7 (clone L243) is a monoclonal antibody conjugated with APC-Cy7 fluorochrome. It is designed to detect the expression of HLA-DR, a major histocompatibility complex (MHC) class II cell surface receptor, on cells.

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Lab products found in correlation

Alexa fluor 488 antibody, by Thermo Fisher Scientific (2 mentions) Anti cd3 bv421 clone sp34 2, by BD (2 mentions) Anti cd16 bv605 clone sg8, by BD (2 mentions) Anti cd4 bv711 clone l200, by BD (2 mentions) Anti cd14 bv786 clone m5e2, by BD (2 mentions) Anti cd123 percp cy5.5 clone 7g3, by BD (2 mentions) Anti cd8 pe cy7 clone sk1, by BD (2 mentions) Anti cd11c alexa700 clone 3.9, by Thermo Fisher Scientific (2 mentions) Anti p100 clone epr18756, by Abcam (2 mentions) 4 laser fortessa flow cytometer, by BD (2 mentions) Anti cd20 pe cf594 clone 2h7, by BD (2 mentions) Cytofix cytoperm kit, by BD (2 mentions)

Close spelling variants of this product

Anti-HLA-DR APC (clone L243) HLA-DR (L243) APC-Cy7 APC‐HLA‐DR clone L243 Anti-HLA-DR (clone L243, HLA-DR-APC-L243 HLA-DR (clone L243, Anti-HLA-DR-APC-Cy7 HLA-DR-APC-Cy7 Anti-HLA-DR-APC HLA-DR-APC

2 protocols using anti hla dr apc cy7 clone l243

1

Multiparameter Flow Cytometric Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole blood collected in Cyto-Chex® blood collection tubes (Myriad RBM) was added to ACK lysis buffer to remove red blood cells, then washed with PBS and resuspended in a staining cocktail including anti-CD3 BV421 (clone SP34-2; BD Biosciences), anti-CD16 BV605 (clone SG8; BD Biosciences), anti-CD4 BV711 (clone L200; BD Biosciences), anti-CD14 BV786 (clone M5E2; BD Biosciences), anti-CD123 PerCP-Cy5.5 (clone 7G3; BD Biosciences), anti-CD20 PE-CF594 (clone 2H7; BD Biosciences), anti-CD8 PE-Cy7 (clone SK1; BD Biosciences), anti-CD11c Alexa700 (clone 3.9; Ebioscience), and anti-HLA-DR APC-Cy7 (clone L243; BD Biosciences). Following surface staining, cells were washed twice with phosphate-buffered saline (PBS), then permeabilized using the Cytofix/Cytoperm kit (BD Biosciences, kit used as directed), and stained intracellularly with anti-p100 (clone EPR18756; Abcam). Cells were washed twice and stained with a secondary chicken anti-rabbit Alexa Fluor 488 antibody (Invitrogen). Samples were acquired using a 4-laser Fortessa™ flow cytometer (BD Biosciences) and analyzed with FlowJo™ software (version 9.7.6, Treestar).
Nixon C.C., Mavigner M., Sampey G.C., Brooks A.D., Spagnuolo R.A., Irlbeck D.M., Mattingly C., Ho P.T., Schoof N., Cammon C.G., Tharp G.K., Kanke M., Wang Z., Cleary R.A., Upadhyay A.A., De C., Wills S.R., Falcinelli S.D., Galardi C., Walum H., Schramm N.J., Deutsch J., Lifson J.D., Fennessey C.M., Keele B.F., Jean S., Maguire S., Liao B., Browne E.P., Ferris R.G., Brehm J.H., Favre D., Vanderford T.H., Bosinger S.E., Jones C.D., Routy J.P., Archin N.M., Margolis D.M., Wahl A., Dunham R.M., Silvestri G., Chahroudi A, & Garcia J.V. (2020). Systemic HIV and SIV latency reversal via non-canonical NF-κB signalling in vivo. Nature, 578(7793), 160-165.
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2

Multiparameter Flow Cytometric Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole blood collected in Cyto-Chex® blood collection tubes (Myriad RBM) was added to ACK lysis buffer to remove red blood cells, then washed with PBS and resuspended in a staining cocktail including anti-CD3 BV421 (clone SP34-2; BD Biosciences), anti-CD16 BV605 (clone SG8; BD Biosciences), anti-CD4 BV711 (clone L200; BD Biosciences), anti-CD14 BV786 (clone M5E2; BD Biosciences), anti-CD123 PerCP-Cy5.5 (clone 7G3; BD Biosciences), anti-CD20 PE-CF594 (clone 2H7; BD Biosciences), anti-CD8 PE-Cy7 (clone SK1; BD Biosciences), anti-CD11c Alexa700 (clone 3.9; Ebioscience), and anti-HLA-DR APC-Cy7 (clone L243; BD Biosciences). Following surface staining, cells were washed twice with phosphate-buffered saline (PBS), then permeabilized using the Cytofix/Cytoperm kit (BD Biosciences, kit used as directed), and stained intracellularly with anti-p100 (clone EPR18756; Abcam). Cells were washed twice and stained with a secondary chicken anti-rabbit Alexa Fluor 488 antibody (Invitrogen). Samples were acquired using a 4-laser Fortessa™ flow cytometer (BD Biosciences) and analyzed with FlowJo™ software (version 9.7.6, Treestar).
Nixon C.C., Mavigner M., Sampey G.C., Brooks A.D., Spagnuolo R.A., Irlbeck D.M., Mattingly C., Ho P.T., Schoof N., Cammon C.G., Tharp G.K., Kanke M., Wang Z., Cleary R.A., Upadhyay A.A., De C., Wills S.R., Falcinelli S.D., Galardi C., Walum H., Schramm N.J., Deutsch J., Lifson J.D., Fennessey C.M., Keele B.F., Jean S., Maguire S., Liao B., Browne E.P., Ferris R.G., Brehm J.H., Favre D., Vanderford T.H., Bosinger S.E., Jones C.D., Routy J.P., Archin N.M., Margolis D.M., Wahl A., Dunham R.M., Silvestri G., Chahroudi A, & Garcia J.V. (2020). Systemic HIV and SIV latency reversal via non-canonical NF-κB signalling in vivo. Nature, 578(7793), 160-165.
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