Whatman fta elute card

Manufactured by GE Healthcare

Sourced in United States

Whatman™ FTA™ Elute Cards are a collection of paper-based collection media designed for the storage and transport of biological samples. The cards are pre-treated with a proprietary chemical formula that stabilizes and preserves nucleic acids, proteins, and other biomolecules within the sample matrix.

Automatically generated - may contain errors

Lab products found in correlation

Ethylene diamine tetraacetic acid (edta), by Terumo (1 mentions) Kraken software, by Biosearch Technologies (1 mentions)

Close spelling variants of this product

Whatman FTA cards FTA cards Whatman FTA

6 protocols using whatman fta elute card

Donkey Blood Sampling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were obtained from 198 donkeys in a local market in West Omdurman, Khartoum State
(Fig. 1Fig. 1.

Map of Sudan showing the sampling location in West Omdurman, Omdurman city,
Khartoum State.

), after obtaining consent from the donkey owners. Apparently healthy donkeys, that
did not present with typical symptoms or health complaints as indicated by their owners,
were selected randomly for sampling. Briefly, 8 ml of blood was drawn
from the jugular vein; 3 ml was stored in vacutainer tubes with EDTA
(Terumo, Tokyo, Japan) for DNA extraction, and 5 ml was stored in plain
vacutainers (Terumo) for serum separation. Sera were separated by centrifugation into
1.5-ml tubes and kept at −20°C until use. Genomic DNA of each sample
was extracted from whole blood after loading onto Whatman™ FTA™ Elute Cards (GE
Healthcare, Chicago, IL, USA), according to the manufacturer’s instructions. Permission
for this study was obtained according to the standards of animal experimentation at
Obihiro University of Agriculture and Veterinary Medicine (Approval No. 29-2, 18-18,
19-19).

ELATA A., MOSSAAD E., SATTI R., MATAR N., OHARI Y., XUAN X., INOUE N, & SUGANUMA K. (2020). Serological and molecular detection of selected hemoprotozoan parasites in donkeys in West Omdurman, Khartoum State, Sudan. The Journal of Veterinary Medical Science, 82(3), 286-293.

+ Open protocol

+ Expand

Blood Spot Collection on FTA Elute Cards

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood spots were collected on Whatman® FTA® Elute cards (GE Healthcare Life Sciences) as per manufacturer's protocols.

Zurita M., Martignette L., Barrera J., Carrie M., Piscatelli H., Hangman A., Brake D., Neilan J., Petrik D, & Puckette M. (2021). Detection of African swine fever virus utilizing the portable MatMaCorp ASF detection system. Transboundary and Emerging Diseases, 69(5), 2600-2608.

+ Open protocol

+ Expand

Malaria Prevalence Study in Ghana

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasmodium falciparum-infected blood smear positive samples from patients in Ghana used for this study were collected on Whatman^® FTA Elute cards (GE Lifesciences, MA, USA) at Kpone Katamanso District Hospital in the Greater Accra Region of Ghana, a malaria endemic country which recorded about 10 million malaria cases in the year 2015. The study site was developed for multidisciplinary malaria studies by the University of Ghana School of Public Health [13 (link)]. Kpone Katamanso district is a peri-urban district located in the coastal-savannah zone that occupies an area of 215.4 km² and has an estimated population of 50,225. While malaria transmission intensity of the region varies seasonally, infections are acquired year-round. Study participants were 1 month to 16 years old children and asymptomatic adults who were screened in the Kpone Katamanso District Hospital.

Grabias B., Essuman E., Quakyi I.A, & Kumar S. (2019). Sensitive real-time PCR detection of Plasmodium falciparum parasites in whole blood by erythrocyte membrane protein 1 gene amplification. Malaria Journal, 18, 116.

+ Open protocol

+ Expand

Fecal T. gondii DNA Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whatman™ FTA™ Elute Cards (GE Healthcare Life Sciences, MA, USA) were used for room temperature collection, preservation, and purification of nucleic acids from samples for detection. Briefly, the faeces samples were treated with 50 μL SEMP (containing 1 M Tris-HCl, 700 mM EDTA, 10% SDS, β-mercaptoethanol and equilibrium phenol) (Solarbio, China) in a 1.5 mL nuclease-free tube (Cao et al., 2020 (link)). After full homogenization, 0.2 mg/mL chitinase was added at room temperature for 30 min to remove the impact of spores for DNA extraction. Nucleic acid was absorbed onto FTA elute cards, which were cut into 2 mm discs using a sterile 2 mm Harris punch (WB100039) and a cutting mat, and 2 mm discs were punched out and placed into the homogenate, stirring the nucleic acid adsorption material with tweezers to fully wet it. The FTA discs were washed twice, and nucleic acids were eluted with 20 μL sterile water to obtain T. gondii DNA. The concentration of nucleic acids was measured using a NanoDrop2000 and stored at -80°C. Finally, 1 μL of eluant was added to the qLAMP and visual LAMP systems for detection.

Cao Z., Zhang K., Yin D., Zhang Q., Yu Y., Wen J, & Ni H. (2022). Clinical validation of visual LAMP and qLAMP assays for the rapid detection of Toxoplasma gondii. Frontiers in Cellular and Infection Microbiology, 12, 1024690.

+ Open protocol

+ Expand

Genomic DNA Extraction and Genotyping

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

10 μL of blood was transferred onto a Whatman FTA Elute card (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) and allowed to dry overnight. The DNA extraction procedure followed the manufacturer's instructions. Genotyping was completed on the 90 SNP panel (MHC-J6 to MHC-178) using KASP (LGC Biosearch Technologies, Middlesex, UK) genotyping procedures (subset of the 101 SNP panel) described in Fulton et al. (2016b) (link). Briefly, the KASP technology uses PCR-based single SNP assays with allele-specific competitive amplification, and fluorescence-based end-point reads (Semagn et al., 2013 (link)). Any PCR-based technology will work for detection of the alleles, if the primer sequences are known. Details, including the specific primers for each assay, are given in the study by Fulton et al., 2016b (link). Genotypes were determined using Kraken software (LGC Biosearch Technologies, Middlesex, UK).

Tarrant K.J., Lopez R., Loper M, & Fulton J.E. (2020). Assessing MHC-B diversity in Silkie chickens. Poultry Science, 99(5), 2337-2341.

+ Open protocol

+ Expand

DNA Extraction from Biological Fluids

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole blood, saliva, and urine samples were each collected from healthy donors according to protocols approved by IRB of Georgia Institute of Technology. To purify DNA by the FTA card, Whatman FTA Elute Card (GE Healthcare, UK) was used according to manufacturer-provided protocol. Samples (50 μl) were deposited onto the FTA cards within the circular spot, and the samples were dried thoroughly for at least 3 hours at room temperature. The dried samples on the FTA card were punched out using a 3-mm biopsy punch, and each of them was placed into sterile microcentrifuge tubes for the following washing steps. The punched discs were then triple-washed in 500 μl of DI water through vortexing and centrifugation. Following the final wash, the extracted water was aspirated from the tube, and the washed discs were directly used for qPCR analysis.

Lee D., Ozkaya-Ahmadov T., Chu C.H., Boya M., Liu R, & Sarioglu A.F. (2021). Capillary flow control in lateral flow assays via delaminating timers. Science Advances, 7(40), eabf9833.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!