SH-SY5Y (ECACC, Salisbury, Wiltshire, United Kingdom), HCT-15, HCT116, HEK293, HEK293T and MCF-7 cells were cultured in DMEM/F-12 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum. To obtain normal human matured neuronal cells in a culture system, ReproNeuro, a neuron progenitor derived from human iPS cells, was purchased from ReproCELL (Yokohama, Japan) and maintained in ReproNeuro maturation medium for 14 days according to the manufacturer’s instructions. Carbonyl cyanide m-chlorophenyl hydrazone (CCCP), 6-hydroxydopamine hydrobromide (6-OHDA), cadmium chloride (CdCl2), rotenone, tert-Butylhydroquinone (tBHQ), sulforaphane and N-acetylcysteine (NAC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Paraquat, caffeine and curcumin were purchased from Wako Chemicals (Osaka, Japan). The antibodies used were as follows: an antibody against PINK1 (rabbit monoclonal, 6946), an antibody against NRF2 (rabbit monoclonal, 12721), horseradish peroxidase (HRP)-conjugated anti-mouse and anti-rabbit secondary antibodies (Cell Signaling Technologies, Danvers, MA, USA), and an antibody against Tubulin (mouse monoclonal, T5168; Sigma-Aldrich).
Reproneuro
Manufactured by ReproCELL
Sourced in Japan
ReproNeuro is a specialized laboratory equipment designed for research and development in the field of neuroscience. The core function of ReproNeuro is to facilitate the cultivation and study of neural cells, tissues, and organoids in a controlled and precise environment.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase hrp conjugated anti mouse and anti rabbit secondary antibodies, by Cell Signaling Technology (2 mentions)
Caffeine, by Fujifilm (1 mentions)
Curcumin, by Fujifilm (1 mentions)
N acetylcysteine nac, by Merck Group (1 mentions)
Paraquat, by Fujifilm (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
Ki16425, by Merck Group (1 mentions)
Sc 47778, by Cell Signaling Technology (1 mentions)
Gw9662, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
2 protocols using reproneuro
1
Neuronal Cell Culture and Toxicity Assays
2
Mature Human Neuron Differentiation from iPSCs
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To obtain mature human matured neurons in a culture system, ReproNeuro, a neuron progenitor derived from human iPSCs, was purchased from ReproCELL (Yokohama, Japan) and maintained in ReproNeuro maturation medium for 14 days according to the manufacturer's instructions. All assays were performed on cells between passages 3 and 12 and were repeated at least 3 times in duplicate or triplicate. LPA (18:1) was purchased from Cayman Chemical (Ann Arbor, MI); LPA was dissolved in phosphate-buffered saline containing 0.1% lipid-free bovine serum albumin (Sigma-Aldrich, MO) to generate a 10-mM stock solution. GW9662 and Ki16425 were purchased from Sigma-Aldrich (St. Louis, MO).
We used antibodies against PPARγ (rabbit monoclonal, sc-7196) and KLF9 (rabbit monoclonal, GTX129316), horseradish peroxidase (HRP)-conjugated anti-mouse and antirabbit secondary antibodies (Cell Signaling Technologies, Danvers, MA), and an antibody against β-actin (mouse monoclonal, sc-47778).
We used antibodies against PPARγ (rabbit monoclonal, sc-7196) and KLF9 (rabbit monoclonal, GTX129316), horseradish peroxidase (HRP)-conjugated anti-mouse and antirabbit secondary antibodies (Cell Signaling Technologies, Danvers, MA), and an antibody against β-actin (mouse monoclonal, sc-47778).
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