Log In Sign up
The largest database of trusted experimental protocols

Hrp conjugated α tubulin

Manufactured by Cell Signaling Technology
Visit Supplier

HRP conjugated α–Tubulin is a laboratory reagent used for the detection and visualization of α-tubulin, a key structural component of microtubules, in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Ecl western blotting analysis system, by GE Healthcare (4 mentions) Nupage transfer buffer, by Thermo Fisher Scientific (4 mentions) Mini protean 3 cell system, by Thermo Fisher Scientific (4 mentions) 0.45 μm nitrocellulose membrane, by Bio-Rad (4 mentions) Membrane blocking solution, by Thermo Fisher Scientific (4 mentions) Phospho smad2 3, by Cell Signaling Technology (2 mentions) Biotinylated smad2 3, by Cell Signaling Technology (2 mentions) Tbx20, by Merck Group (2 mentions) Cdkn1a, by Cell Signaling Technology (2 mentions) Rabbit anti lmna, by Santa Cruz Biotechnology (2 mentions) Mouse anti lmna, by Santa Cruz Biotechnology (2 mentions) Camk2d, by Abcam (2 mentions) Pdgfrb, by Cell Signaling Technology (2 mentions)

Spelling variants of this product

α-tubulin (597 citations) α-tubulin-HRP (2 citations) Tubulin-HRP (2 citations) Anti-α-tubulin-HRP conjugated antibody (2 citations)

4 protocols using hrp conjugated α tubulin

1

Western Blot Analysis of Protein Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following SDS-PAGE, proteins were transferred to 0.45 μm nitrocellulose membranes (Bio-Rad) using a mini Bio-Rad Mini PROTEAN 3 Cell system in NuPAGE transfer buffer (Life technologies). The membrane was then blocked in Membrane Blocking Solution (Life technologies) and incubated with primary antibody overnight at 4 °C. Blots were incubated with the appropriate secondary antibodies for 1 h at room temperature and visualized using ECL Western Blotting Analysis System (GE Healthcare). Primary antibodies used were biotinylated SMAD2/3 (Cell Signaling), phospho-SMAD2/3 (Cell Signaling), CDKN1A (Cell Signaling), TBX20 (Sigma Aldrich), and HRP conjugated α–Tubulin (Cell Signaling). A detailed list of antibodies used is shown in Supplementary Table 10.
Kodo K., Ong S.G., Jahanbani F., Termglinchan V., Hirono K., InanlooRahatloo K., Ebert A.D., Shukla P., Abilez O.J., Churko J.M., Karakikes I., Jung G., Ichida F., Wu S.M., Snyder M.P., Bernstein D, & Wu J.C. (2016). iPSC-derived cardiomyocytes reveal abnormal TGFβ signaling in left ventricular non-compaction cardiomyopathy. Nature cell biology, 18(10), 1031-1042.
+ Open protocol
+ Expand
2

Western Blot Analysis of Protein Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following SDS-PAGE, proteins were transferred to 0.45 μm nitrocellulose membranes (Bio-Rad) using a mini Bio-Rad Mini PROTEAN 3 Cell system in NuPAGE transfer buffer (Life technologies). The membrane was then blocked in Membrane Blocking Solution (Life technologies) and incubated with primary antibody overnight at 4 °C. Blots were incubated with the appropriate secondary antibodies for 1 h at room temperature and visualized using ECL Western Blotting Analysis System (GE Healthcare). Primary antibodies used were biotinylated SMAD2/3 (Cell Signaling), phospho-SMAD2/3 (Cell Signaling), CDKN1A (Cell Signaling), TBX20 (Sigma Aldrich), and HRP conjugated α–Tubulin (Cell Signaling). A detailed list of antibodies used is shown in Supplementary Table 10.
Kodo K., Ong S.G., Jahanbani F., Termglinchan V., Hirono K., InanlooRahatloo K., Ebert A.D., Shukla P., Abilez O.J., Churko J.M., Karakikes I., Jung G., Ichida F., Wu S.M., Snyder M.P., Bernstein D, & Wu J.C. (2016). iPSC-derived cardiomyocytes reveal abnormal TGFβ signaling in left ventricular non-compaction cardiomyopathy. Nature cell biology, 18(10), 1031-1042.
+ Open protocol
+ Expand
3

Western Blot Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following SDS–PAGE, proteins were transferred to 0.45 μm nitrocellulose membranes (Bio-Rad) using a mini Bio-Rad Mini PROTEAN 3 Cell system in NuPAGE transfer buffer (Life technologies). The membrane was then blocked in Membrane Blocking Solution (Life technologies) and incubated with primary antibodies overnight at 4 °C. Blots were incubated with the appropriate secondary antibodies for 1 hr at room temperature and visualized using the ECL Western Blotting Analysis System (GE Healthcare). Primary antibodies used were mouse anti-LMNA (Santa Cruz), rabbit anti-LMNA (Santa Cruz), CAMK2D (Abcam), PDGFRB (Cell Signaling), RYR2 (Abcam), phospho-RYR2 (Donald M. Bers lab), and HRP-conjugated α−tubulin (Cell Signaling).
Lee J., Termglinchan V., Diecke S., Itzhaki I., Lam C.K., Garg P., Lau E., Greenhaw M., Seeger T., Wu H., Zhang J.Z., Chen X., Gil I.P., Ameen M., Sallam K., Rhee J.W., Churko J., Chaudhary R., Chour T., Wang P.J., Snyder M.P., Chang H.Y., Karakikes I, & Wu J.C. (2019). Activation of PDGF Pathway Links LMNA Mutation to Dilated Cardiomyopathy. Nature, 572(7769), 335-340.
+ Open protocol
+ Expand
4

Western Blot Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following SDS–PAGE, proteins were transferred to 0.45 μm nitrocellulose membranes (Bio-Rad) using a mini Bio-Rad Mini PROTEAN 3 Cell system in NuPAGE transfer buffer (Life technologies). The membrane was then blocked in Membrane Blocking Solution (Life technologies) and incubated with primary antibodies overnight at 4 °C. Blots were incubated with the appropriate secondary antibodies for 1 hr at room temperature and visualized using the ECL Western Blotting Analysis System (GE Healthcare). Primary antibodies used were mouse anti-LMNA (Santa Cruz), rabbit anti-LMNA (Santa Cruz), CAMK2D (Abcam), PDGFRB (Cell Signaling), RYR2 (Abcam), phospho-RYR2 (Donald M. Bers lab), and HRP-conjugated α−tubulin (Cell Signaling).
Lee J., Termglinchan V., Diecke S., Itzhaki I., Lam C.K., Garg P., Lau E., Greenhaw M., Seeger T., Wu H., Zhang J.Z., Chen X., Gil I.P., Ameen M., Sallam K., Rhee J.W., Churko J., Chaudhary R., Chour T., Wang P.J., Snyder M.P., Chang H.Y., Karakikes I, & Wu J.C. (2019). Activation of PDGF Pathway Links LMNA Mutation to Dilated Cardiomyopathy. Nature, 572(7769), 335-340.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!