Anti p jak2

STZ was purchased from Sigma-Aldrich (St. Louis, MO, USA). Sitagliptin (Januvia) was purchased from Merck & Co., Inc. (Whitehouse, NJ, USA). The inflammatory biomarkers TNFα and IL-6 and DN biomarkers urea, BUN, and creatinine were measured using specific enzyme-linked immunosorbent assay (ELISA) kits from Abcam (Biotechnology Inc., Cambridge, UK). The rabbit polyclonal PTP1B antibody and anti-JAK2, anti-P-JAK2, anti-STAT3, and anti-P-STAT3 antibodies were obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Both β-actin, the housekeeping loading control antibody, and other associated primary antibodies were bought from Abcam (Biotechnology Inc., Cambridge, UK). Anti-rabbit, anti-mouse, anti-goat horseradish peroxidase (HRP)-conjugated secondary antibodies, glucose assay kit were sourced from Sigma-Aldrich (St. Louis, MO, USA). Different analytical-grade chemicals and reagents were sourced from standard commercial firms.