Following SDS–PAGE, the isolated proteins were transferred onto nitrocellulose membrane using a Bio-Rad wet blot system for 100 min at 100 V. The membranes were blocked with 2% BSA in PBS for overnight. After gently washing, the membranes were incubated with rabbit anti-TG2 antibody (1/2000, Abcam) for 1.5 h at RT. The detector layer was anti-rabbit IgG (1:10000, Santa Cruz Biotechnology). Finally, visualization was performed with DAB (Sigma).21 (link),22 (link)
Rabbit anti tg2 antibody
Manufactured by Abcam
Rabbit anti-TG2 antibody is a laboratory reagent used to detect and/or quantify transglutaminase 2 (TG2) in biological samples. It is a polyclonal antibody raised in rabbits against the TG2 protein.
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Lab products found in correlation
3 protocols using rabbit anti tg2 antibody
1
Western Blot Protein Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
Following SDS–PAGE, the isolated proteins were transferred onto nitrocellulose membrane using a Bio-Rad wet blot system for 100 min at 100 V. The membranes were blocked with 2% BSA in PBS for overnight. After gently washing, the membranes were incubated with rabbit anti-TG2 antibody (1/2000, Abcam) for 1.5 h at RT. The detector layer was anti-rabbit IgG (1:10000, Santa Cruz Biotechnology). Finally, visualization was performed with DAB (Sigma).21 (link),22 (link)
+ Expand
Following SDS–PAGE, the isolated proteins were transferred onto nitrocellulose membrane using a Bio-Rad wet blot system for 100 min at 100 V. The membranes were blocked with 2% BSA in PBS for overnight. After gently washing, the membranes were incubated with rabbit anti-TG2 antibody (1/2000, Abcam) for 1.5 h at RT. The detector layer was anti-rabbit IgG (1:10000, Santa Cruz Biotechnology). Finally, visualization was performed with DAB (Sigma).21 (link),22 (link)
2
Western Blot Protein Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
Following SDS–PAGE, the isolated proteins were transferred onto nitrocellulose membrane using a Bio-Rad wet blot system for 100 min at 100 V. The membranes were blocked with 2% BSA in PBS for overnight. After gently washing, the membranes were incubated with rabbit anti-TG2 antibody (1/2000, Abcam) for 1.5 h at RT. The detector layer was anti-rabbit IgG (1:10000, Santa Cruz Biotechnology). Finally, visualization was performed with DAB (Sigma).21 (link),22 (link)
+ Expand
Following SDS–PAGE, the isolated proteins were transferred onto nitrocellulose membrane using a Bio-Rad wet blot system for 100 min at 100 V. The membranes were blocked with 2% BSA in PBS for overnight. After gently washing, the membranes were incubated with rabbit anti-TG2 antibody (1/2000, Abcam) for 1.5 h at RT. The detector layer was anti-rabbit IgG (1:10000, Santa Cruz Biotechnology). Finally, visualization was performed with DAB (Sigma).21 (link),22 (link)
3
Western Blot Protein Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
Following SDS–PAGE, the isolated proteins were transferred onto nitrocellulose membrane using a Bio-Rad wet blot system for 100 min at 100 V. The membranes were blocked with 2% BSA in PBS for overnight. After gently washing, the membranes were incubated with rabbit anti-TG2 antibody (1/2000, Abcam) for 1.5 h at RT. The detector layer was anti-rabbit IgG (1:10000, Santa Cruz Biotechnology). Finally, visualization was performed with DAB (Sigma).21 (link),22 (link)
+ Expand
Following SDS–PAGE, the isolated proteins were transferred onto nitrocellulose membrane using a Bio-Rad wet blot system for 100 min at 100 V. The membranes were blocked with 2% BSA in PBS for overnight. After gently washing, the membranes were incubated with rabbit anti-TG2 antibody (1/2000, Abcam) for 1.5 h at RT. The detector layer was anti-rabbit IgG (1:10000, Santa Cruz Biotechnology). Finally, visualization was performed with DAB (Sigma).21 (link),22 (link)
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