Waters acquity tunable uv detector

Twenty-one amino acids were separated and quantified using the Waters ACQUITY UPLC analytical system controlled by the Empower II software (Waters). The amino acids were derivatized using AccQ Tag Ultra reagent (6-aminoquinolyl-N-hydroxysuccinimidyl carbamate). The derivatives were separated on a 2.1 × 100 mm AccQ Tag Ultra column (Waters) and detected with Waters ACQUITY Tunable UV detector at 260 nm under the chromatographic conditions described in Cohen (2000). Peak identity and amino acid quantity were determined by comparison to a standard mix containing the 21 amino acids. Results from amino acid determination were expressed as concentrations on DW basis (µmol g⁻¹ DW).

Amino acids were derivatized using the AccQ Tag Ultra reagent (6-aminoquinolyl-N-hydroxysuccinimidyl carbamate) and separated on an AccQ Tag Ultra column (2.1 mm × 100 mm). The 21 amino acids were detected with Waters ACQUITY Tunable UV detector (Waters) at 260 nm under previously described chromatographic conditions (Cohen, 2000 ). The Waters ACQUITY UPLC analytical system was controlled by the Empower II software. Peak identity and quantity were determined by comparison to analytical standards (Supplementary Table S1), and results were expressed as concentrations on dry weight basis (μmol g^-1 DW).