Agilent 6540 q tof ms system

The quantitative analysis of the five analytes was carried out using an Agilent 6540 Q-TOF–MS system (Agilent Technologies, Santa Clara, CA, USA) equipped with a jet stream ESI interface. The MS data were obtained in a MS scan mode. Mass spectra were recorded from m/z 50 to 800 in positive ionization mode. The optimized mass analysis conditions were as follows: drying gas (N₂) flow rate, 10 L min⁻¹; drying gas temperature, 350 °C; nebulizer, 310 kPa; sheath gas temperature, 250 °C; capillary voltage, 4000 V; fragmentor voltage, 140 V; nozzle voltage, 500 V; octopole RF voltage, 750 V. All the operations and data analysis were controlled using an integrated software system including Symbiosis Pico in Analyst™ version 1.2.00 (Spark Holland) and MassHunter B.04.00 software (Agilent Technologies, USA).

HPLC analysis was carried out on an Agilent 1200 series equipped with a diode array detector (Agilent Technologies, USA). Chromatographic separation was performed on a C₁₈ column (4.6 × 150 mm, 3.5 µm, Zorbax eclipse plus, Agilent USA) at 30°C with a guard column (4.6 × 12.5 mm, 5 µm, Zorbax eclipse plus). The mobile phase consisted of H₂O (solvent A) and ACN (solvent B) with 0.1% (v/v) formic acid, respectively. The gradient program was as follows: 0‐19 min, 10‐46% B; 19‐20 min, 46‐10% B. A post‐run equilibrium time of 3 min was used for all samples. The flow rate was set at 1 mL/min with ultraviolet (UV) detection at 350 nm. The T splitter gave a flow rate of 0.25 mL/min toward the MS detector.
Mass spectrometry was performed using an Agilent 6540 Q‐TOF‐MS system (Agilent Corp., USA) equipped with a jet stream ESI interface. The TOF/MS system was operated in both negative and positive ion modes. Mass spectra were recorded over the mass range m/z 50‐1100. The mass analysis conditions were set as follows: drying gas (N₂) flow rate, 10 L/min; nebulizer, 40 psi; drying gas temperature, 350°C; sheath gas temperature, 350°C; capillary voltage, 3500 V; fragmentor voltage, 120 V.