Applied biosystems geneamp pcr system 9700
The Applied Biosystems GeneAmp PCR System 9700 is a thermal cycler designed for performing polymerase chain reaction (PCR) experiments. It has the capability to precisely control temperature and timing of the thermal cycling process, which is essential for DNA amplification and analysis.
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13 protocols using applied biosystems geneamp pcr system 9700
Isolation and qPCR Analysis of Macrophage RNA
Transcriptomic Analysis of Decidual and Myometrial Tissues
Amplification and Sequencing of MC1R and KIT Genes
Investigating QS Regulation in P. aeruginosa
Genetic Profiling of Tumor Specimens
Genetic Analysis of MADD Patients
Synthesizing cDNA from RNA Samples
SNP Genotyping for Sample Identification
Sequencing of Tumor Driver Genes
DNA Profiling of Buccal Swab Samples
PowerPlex® ESI kit (Promega Corp.) was used for further analysis. Similar amounts of DNA were used in all PCR reactions. Amplification was carried out as described previously (12 ). The total reaction volume was 10 μL. Additionally, PowerPlex®Y23 kit (Promega Corp.) was used to simultaneously amplify 23 Y-STR loci according to manufacturer’s recommendations (13 ). PCR amplification was carried out by the Applied Biosystems® GeneAmp® PCR System 9700 (Life Technologies) according to manufacturer’s recommendations. Electrophoresis of the amplification products was performed on an ABI PRISM 310 Genetic Analyzer (Applied Biosystems). The raw data were compiled and analyzed using 310 Data Collection Software and GeneMapperTM 3.2.
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