Five-ALA (Medac GmbH, Wedel, Germany) was administered 4 to 6 h prior to surgery at a dose of 20 mg/kg body weight per os. Leica M720 OH5 (Leica Microsystems, Wetzlar, Germany) microscope was routinely used. Intraoperatively, 5-ALA regions of interest were defined under violet–blue illumination (Blue 400 filters). Electrophysiological monitoring (Nicolet Endeavor CR, Cardinal Health, Dublin, Ireland) and intraoperative neuronavigation were used in all cases. According to the surgeon’s request, CUSA (Integra Life Sciences, Princeton, NJ, USA) was employed.
Leica m720 oh5
Manufactured by Leica Microsystems
Sourced in Germany
The Leica M720 OH5 is a high-performance optical microscope designed for clinical and research applications. It features a modular design and offers a range of objective lenses, illumination systems, and accessories to meet the specific needs of various users.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using leica m720 oh5
1
5-ALA Guided Neurosurgical Procedure
2
Detailed Neurosurgical Procedure Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All surgeries were performed with an OPMI Pentero (Carl Zeiss) or a Leica M720 OH5 (Leica Microsystems) microscope. Electrophysiological monitoring (Nicolet Endeavor CR, Cardinal Health) and a neuronavigation system were used when appropriate. A Cavitron ultrasonic surgical aspirator was used in most procedures, according to the surgeon's request.
3
Intraoperative 5-ALA Guided Tumor Sampling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three to 5 hours before surgery, 5-ALA (20 mg/kg of body weight; Medac GmbH) was administered orally to patients. During surgery, 3 different tumor sites were visualized based on the presence of red-violet fluorescence, following excitation with ultraviolet blue light (FL 400 filter, Leica M720 OH5, Leica Microsystems).
Sampling sites were confirmed via intraoperative neuronavigation and classified as follows: 1) The ALA+ area is analogous to the enhancing area in neuronavigation and corresponds to the tumor core, with lava-like red fluorescence. 2) The ALA PALE area is analogous to the tumoral boundaries surrounding the enhancing area and corresponds to the tumor inner periphery, with pale red fluorescence. 3) The tumor outer periphery, without fluorescence, is the ALA-area and is analogous to the nonenhancing areas distant from the tumor (Fig. 2).
For each sampling site, a few cubic millimeters of tissue was collected and preserved in DMEM (Invitrogen) at 4°C before processing.
Sampling sites were confirmed via intraoperative neuronavigation and classified as follows: 1) The ALA+ area is analogous to the enhancing area in neuronavigation and corresponds to the tumor core, with lava-like red fluorescence. 2) The ALA PALE area is analogous to the tumoral boundaries surrounding the enhancing area and corresponds to the tumor inner periphery, with pale red fluorescence. 3) The tumor outer periphery, without fluorescence, is the ALA-area and is analogous to the nonenhancing areas distant from the tumor (Fig. 2).
For each sampling site, a few cubic millimeters of tissue was collected and preserved in DMEM (Invitrogen) at 4°C before processing.
4
Multimodal Intraoperative Imaging and Navigation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Intraoperative MRI was performed in an iMRI suite (IMRIS Visius Surgical Theater, IMRIS Inc.) with a modified, ceiling-mounted, 1.5-T movable magnet (Espree, Siemens Medical Systems), as described in the work by Chen et al. 1 Electrophysiological monitoring (Nicolet Endeavor CR, Cardinal Health) using platinum needles, which can stay in place during scanning, and careful avoidance of cable-to-skin contact; BrainLab neuronavigation (Vector-Vision compact system and Curve); and high-resolution ultrasound (Acuson Antares, Siemens AG) were used in almost all cases. All surgeries were performed with an OPMI Pentero (Carl Zeiss) or a Leica M720 OH5 (Leica Microsystems) microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!