Cpt 1a antibody

The diets used in the study were a Teklad custom research diet (catalog # TD.06414) with adjusted calorie diet (60/Fat) containing 18.4% protein, 21.3% carbohydrate and 60.3% fat (referred to herein as high fat diet, HFD), and an isocaloric normal diet 7022 (29% protein, 56% carbohydrate and 15% fat) (referred to herein as normal diet, ND).. Both diets were purchased from Harlan, Indianapolis, IN and contained similar levels of mineral mix AIN-93G-MX (94046) and vitamin mix AIN93-VX (94047). Compound C21 (97% pure) was custom synthesized. The following reagents were purchased from Abcam, MA: plasma free fatty acid quantification assay kit (catalog # ab65341); leptin mouse ELISA kit (catalog # ab100718); CPT-1A antibody (catalog # ab128568); uncoupling protein-1 antibody (catalog # ab10983). The triglyceride quantification assay kit catalog # K622-100 was purchased from Biovision and ultra-sensitive mouse insulin measuring ELISA kit was purchased from Crystal Chem Inc., Downers Grove, IL. The Estradiol EIA kit (catalog # 582251) was purchased from Cayman Chemical, Ann Arbor, MI. The antibodies for AT2R were custom raised from EZ Biolab, Carmel, IN. The anti-β-actin antibody (catalog # 4970) was purchased from Cell Signaling Technology, Danvers, MA.