SEM images were collected using scanning electron microscopes (Miniscope, Hitachi, Tokyo, Japan; JSM-5600LV, JEOL, Tokyo, Japan). Samples were attached to a pedestal with glue or nail polish without chemical fixation and analyzed according to the manufacturer’s instruction.
Miniscope
Manufactured by Hitachi
Sourced in Japan
The Miniscope is a compact and versatile laboratory equipment designed for various scientific applications. It provides high-quality imaging and analysis capabilities in a small form factor. The Miniscope is a reliable tool for researchers and scientists working in various fields, including microscopy, imaging, and sample analysis.
Automatically generated - may contain errors
3 protocols using miniscope
1
Scanning Electron Microscopy Sample Preparation
2
Cultured HFSCs and Mesenchymal Cells for Hair Follicle Regeneration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HFSCs cultured for 14 days were mixed with embryonic mesenchymal
cells (total 2 × 104 cells; HFSCs/mesenchymal cells
= 1:1), suspended in 0.2 mL of Dulbecco’s modified Eagle’s
medium (DMEM; Sigma-Aldrich) supplemented with 10% (v/v) FBS and 1%
(v/v) penicillin-streptomycin + KG2+ mixed in equal volumes,
and seeded into a non-cell-adhesive round-bottom 96-well plate (Primesurface
96U plate; Sumitomo Bakelite, Ltd. Japan). After 3 days of culture,
the spatial cell distribution was examined using a fluorescence microscope
(DP-71, Olympus, Japan).
HFG-like tissue grafts were transplanted
into shallow stab wounds generated on the backs of nude mice under
anesthesia using a 20-G Ophthalmic V-lance (Alcon, Japan). Thereafter,
an ointment was rubbed on the transplantation sites. The mice were
raised for up to 3 weeks under pathogen-free conditions with ad libitum
feeding. Transplanted sites were observed every 2–3 days, and
images of the hair generated were captured using a digital camera
(Tough, Olympus). Hematoxylin and eosin (HE) staining was performed
21 days after the transplantation. The hair cuticles were observed
with a scanning electron microscope (Miniscope; Hitachi, Japan) without
any treatment.
cells (total 2 × 104 cells; HFSCs/mesenchymal cells
= 1:1), suspended in 0.2 mL of Dulbecco’s modified Eagle’s
medium (DMEM; Sigma-Aldrich) supplemented with 10% (v/v) FBS and 1%
(v/v) penicillin-streptomycin + KG2+ mixed in equal volumes,
and seeded into a non-cell-adhesive round-bottom 96-well plate (Primesurface
96U plate; Sumitomo Bakelite, Ltd. Japan). After 3 days of culture,
the spatial cell distribution was examined using a fluorescence microscope
(DP-71, Olympus, Japan).
HFG-like tissue grafts were transplanted
into shallow stab wounds generated on the backs of nude mice under
anesthesia using a 20-G Ophthalmic V-lance (Alcon, Japan). Thereafter,
an ointment was rubbed on the transplantation sites. The mice were
raised for up to 3 weeks under pathogen-free conditions with ad libitum
feeding. Transplanted sites were observed every 2–3 days, and
images of the hair generated were captured using a digital camera
(Tough, Olympus). Hematoxylin and eosin (HE) staining was performed
21 days after the transplantation. The hair cuticles were observed
with a scanning electron microscope (Miniscope; Hitachi, Japan) without
any treatment.
3
Nude Mouse Hair Follicle Transplantation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Under anesthesia,
mfHFGs were transplanted into shallow incisions created on the backs
of the nude mice by using a 20 G ophthalmic V-lance (Alcon, Tokyo,
Japan). The transplantation site was rubbed with ointment. The mice
were maintained under sterile conditions. The transplantation site
was observed every 2–3 days, and images of the generated hair
were captured using a digital camera. The cuticles of the generated
hair were observed under a scanning electron microscope (Miniscope,
Hitachi, Japan) at 5–10 kV.
mfHFGs were transplanted into shallow incisions created on the backs
of the nude mice by using a 20 G ophthalmic V-lance (Alcon, Tokyo,
Japan). The transplantation site was rubbed with ointment. The mice
were maintained under sterile conditions. The transplantation site
was observed every 2–3 days, and images of the generated hair
were captured using a digital camera. The cuticles of the generated
hair were observed under a scanning electron microscope (Miniscope,
Hitachi, Japan) at 5–10 kV.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!