MTAF CD38high and MTAF CD38low cells were grown in a 24-well plate (12 wells for each cell type), under 3D matrix-producing conditions, and CM was collected as described above. CM from 12 wells were collected and pooled together. After CM collection, cell lysate buffer, obtained from Mouse Angiogenesis Profiler Array Kit (ARY015, R&D Systems, Minneapolis, MN), was added to the wells, and lysates were also pooled together. As quality control we vetted that the CM collected effectively induced CD38-dependent tube formation (not shown). Expression of angiogenesis-related proteins, in the cell lysates, was profiled using the Mouse Angiogenesis Profiler Array Kit according to manufacturer’s protocol. The arrays were imaged with Amersham Imager 600 (GE Healthcare Life Sciences). Quantification of signal intensity was performed with ImageJ. Results shown are from two independent experiments. To verify equal amounts of assessed MTAF CD38high and MTAF CD38low proteins, cell lysate GAPDH levels were measured by immunoblotting as described above.
Mouse angiogenesis profiler array kit
Manufactured by R&D Systems
The Mouse Angiogenesis Profiler Array Kit is a multiplex assay designed to detect the relative expression levels of 53 mouse angiogenesis-related proteins simultaneously in a single sample.
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Lab products found in correlation
2 protocols using mouse angiogenesis profiler array kit
1
Comparative Angiogenesis Profiling of CD38 Subpopulations
2
Comparative Angiogenesis Profiling of CD38 Subpopulations
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MTAF CD38high and MTAF CD38low cells were grown in a 24-well plate (12 wells for each cell type), under 3D matrix-producing conditions, and CM was collected as described above. CM from 12 wells were collected and pooled together. After CM collection, cell lysate buffer, obtained from Mouse Angiogenesis Profiler Array Kit (ARY015, R&D Systems, Minneapolis, MN), was added to the wells, and lysates were also pooled together. As quality control we vetted that the CM collected effectively induced CD38-dependent tube formation (not shown). Expression of angiogenesis-related proteins, in the cell lysates, was profiled using the Mouse Angiogenesis Profiler Array Kit according to manufacturer’s protocol. The arrays were imaged with Amersham Imager 600 (GE Healthcare Life Sciences). Quantification of signal intensity was performed with ImageJ. Results shown are from two independent experiments. To verify equal amounts of assessed MTAF CD38high and MTAF CD38low proteins, cell lysate GAPDH levels were measured by immunoblotting as described above.
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