Fetoplacental units were harvested at different dpc, fixed in 10% formalin (Thermo-Fisher, Waltham, MA), dehydrated in a graded series of ethanol (Thermo-Fisher) and xylene (Thermo-Fisher), followed by infiltration of melted paraffin (Thermo-Fisher) at 56°C in an automated processor. The tissues embedded in paraffin were sectioned vertically (the chorionic plate providing the theoretical horizontal plane) at a thickness of 7 μm as described (46 (link)), and tissue sections were mounted on SuperFrost® Plus slides (Thermo-Fisher Scientific). The tissue sections were baked, deparaffined, and rehydrated before being stained with a hematoxylin and eosin (H&E) solution (Sigma-Aldrich, St. Louis, MO) or with a Periodic acid–Schiff stain (PAS) solution (Cat. # 150680, Abeam, Cambridge, MA) as per the manusfecturer’s instructions. The stained tissue sections that were close to the placental midline and exhibited the largest area among the serial sections were imaged under an Olympus BX53 fluorescent microscope (Olympus Corporation of the Americas, Center Valley, PA).
Hematoxylin and eosin h e solution
Manufactured by Merck Group
Hematoxylin and eosin (H&E) solution is a widely used staining combination in histology and pathology laboratories. It consists of two dyes, hematoxylin and eosin, which selectively stain different cellular and tissue structures. The hematoxylin stains nuclei blue or purple, while the eosin stains cytoplasm and other structures pink or red. This combination of stains allows for the visualization and differentiation of various cellular and tissue components under a microscope.
Automatically generated - may contain errors
Lab products found in correlation
Masson s trichrome mt, by Merck Group (2 mentions)
Superfrost plus slide, by Thermo Fisher Scientific (2 mentions)
Xylene, by Thermo Fisher Scientific (1 mentions)
Paraffin, by Thermo Fisher Scientific (1 mentions)
Ethanol, by Thermo Fisher Scientific (1 mentions)
Bx53 fluorescent microscope, by Olympus (1 mentions)
3 protocols using hematoxylin and eosin h e solution
1
Histological Analysis of Fetoplacental Units
2
Lung Histological Assessment Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mouse lung (right) was perfused with 1 mL 10% (v/v) neutral-buffered formalin fixation solution through the trachea; then, the lungs were removed and submerged in formalin buffer. The tissue was paraffinized, cut to 5 μm thickness, and stained with Masson’s trichrome (MT) or hematoxylin and eosin (H&E) solution for the observation of inflammatory cell penetration and collagen fiber formation (Sigma-Aldrich, Seoul, Republic of Korea). The severity degree of inflammation was scored in a double-blind manner by two independent researchers and was determined using a subjective scale of 0–2 described by Lee et al. [8 (link)].
3
Lung Histological Assessment Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mouse lung (right) was perfused with 1 mL 10% (v/v) neutral-buffered formalin fixation solution through the trachea; then, the lungs were removed and submerged in formalin buffer. The tissue was paraffinized, cut to 5 μm thickness, and stained with Masson’s trichrome (MT) or hematoxylin and eosin (H&E) solution for the observation of inflammatory cell penetration and collagen fiber formation (Sigma-Aldrich, Seoul, Republic of Korea). The severity degree of inflammation was scored in a double-blind manner by two independent researchers and was determined using a subjective scale of 0–2 described by Lee et al. [8 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!