Anti γδtcr pe

Manufactured by Thermo Fisher Scientific

The Anti-γδTCR-PE is a laboratory instrument used for the detection and analysis of gamma-delta T cell receptor (γδTCR) expression. It is a flow cytometry reagent that binds specifically to the γδTCR and is conjugated with the fluorescent dye phycoerythrin (PE) for visualization and quantification purposes. This product provides a means to identify and characterize γδT cells within a sample.

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Lab products found in correlation

α tcrβ fitc, by BD (1 mentions) α cd11b pe cy7, by BioLegend (1 mentions) αcd4 fitc, by BD (1 mentions) αcd3 pb, by BioLegend (1 mentions) αcd3 bv421, by BioLegend (1 mentions) αcd11c apc, by BioLegend (1 mentions) Lsr 2, by BD (1 mentions) Anti cd8 apc h7, by Thermo Fisher Scientific (1 mentions) Facs lyse solution, by BD (1 mentions)

2 protocols using anti γδtcr pe

Murine T Cell Immunophenotyping Protocol

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Anti-γδTCR-PE, αTCRβ-APC-eFluor780, αCD11c-FITC, αCD45.1-BV421, αSlamf4-PE, αSlamf4-FITC and αGranzymeB-FITC (eBioscience, San Diego, CA); αCD4-FITC and αTCRβ-FITC (BD Biosciences, San Jose, CA). αCD3-PB, αCD3-BV421, αCD8α-APC, αCD8α-PE-Cy7, αCD8β-PE, αCD8β-PerCP/Cy5.5, αCD4-BV650, αSlamf2-PE, αCD45.2-AlexaFluor 647, αCD45.2 FITC, αCD11b-PECy7, αCD11c-APC, αI-A/I-E-PB, αCCR9-PE, and αα4β7-APC were purchased from Biolegend (San Diego, CA). Data were collected using a BD LSRII and analyzed with FlowJo software (Tree Star, Ashland, OR).

O’Keeffe M.S., Song J.H., Liao G., De Calisto J., Halibozek P.J., Mora J.R., Bhan A.K., Wang N., Reinecker H.C, & Terhorst C. (2015). SLAMF4 is a Negative Regulator of Expansion of Cytotoxic Intra-epithelial CD8+ T Cells That Maintains Homeostasis in the Small Intestine. Gastroenterology, 148(5), 991-1001.e4.

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Longitudinal Analysis of γδ T Cells

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One ml of whole blood was collected in a sodium heparin tube from each volunteer at study day 0, day 38, day 98 and day 154 during the period of the study, which coincided with a clinical follow-up visit day. One hundred fifty μl of whole blood was used for staining with a cocktail of antibodies against: anti-CD3 BV650, anti-CD8 APC-H7, anti-γδTCR PE, anti-Vδ2 FITC purchased from e-bioscience. Red blood cells were lysed using the BD FACS Lyse solution and the cells were washed two times with PBS and acquired on a LSRII flow cytometer equipped with a Blue (488 nm), Red (633 nm) and Violet laser (405 nm). γδT cells (Vδ2+ and Vδ2− populations) were enumerated as a percentage of total CD3 T cells.

Diallo H., Katile A., Kwan J.L., Sissoko M.S., Healy S.A., Doumbo O.K., Duffy P.E, & Zaidi I. (2019). Longitudinal analysis of gamma delta T cell subsets during malaria infections in Malian adults. Malaria Journal, 18, 69.

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