The correlations of protein expressions and localizations between DVL3 and sFRP3 as well as their correlation to pathological and demographic features were analyzed. All individuals were analyzed for the following features: sex, age, DVL3 and sFRP3 protein expression intensities and localizations, and recurrence. Available samples were tested for IDH1 and ATRX mutations. Statistical relevance was tested with Pearson's correlations and Student's t-test depending of the number of cases. The distribution of protein signals on subcellular localization was tested with Mann–Whitney U test (also known as Wilcox rank-sum test (WRS)). This nonparametric test has null hypothesis that two groups of samples originate from the same population, contrary to the alternative hypothesis. In this case, specific population takes on higher values from the other. It can be applied on unknown distributions. Essentially, this test proves that the distribution is opposed to normal distribution. The sum of Wilcox rank was tested with the correction for continuity.
Statistical significance was set at P < 0.05. All statistical evaluations were performed with the SPSS statistical package 14.0 (SPSS Inc., Chicago, IL, USA).
Statistical significance was set at P < 0.05. All statistical evaluations were performed with the SPSS statistical package 14.0 (SPSS Inc., Chicago, IL, USA).