Rat glucagon elisa kit

Blood metabolic parameters were detected under different physiological conditions, i.e., random-fed state and 16 h fasted state. Whole-blood glucose levels were determined using a hand-held glucose monitor (ACCU-CHEK, Roche Diagnostics), and blood was obtained from the tail vein. For serum preparation, the blood was collected into sterile 1.5 mL tubes and was allowed to clot by standing undisturbed at room temperature for 1 h. The samples were centrifuged at 2000×g for 10 min at 4 °C and the resulting supernatant was immediately apportioned into aliquots and stored at − 80 °C. For plasma preparation, the blood was collected into EDTA-treated tubes (BD Vacutainer) and mixed gently. Blood cells were removed by centrifugation at 2000×g for 10 min at 4 °C and the resulting supernatant was immediately apportioned into aliquots and stored at − 80 °C. Serum insulin was measured with Rat Insulin ELISA kit (Mercodia, Sweden). Serum triglyceride and cholesterol levels were measured with LabAssay Triglyceride and LabAssay Cholesterol kits (Wako, Japan), respectively. Serum free fatty acids were measured with a free fatty acid quantification kit (BioVision, USA). Plasma glucagon was measured with Rat Glucagon ELISA Kit (Wako, Japan). All measurements were in accordance with the manufacturer’s instructions.

Serum Tch, TG, HDL, LDL levels were measured by an automatic biochemistry analyzer (Olympus AU5400, Tokyo, Japan). Commercial assay kits were used to determine plasma Glucagon (Rat Glucagon ELISA kit, WAKO, Japan), C-peptide (Rat C-peptide ELISA; ALPCO) levels were measured using ELISA kits. S-Equol was measured by using HPLC method as the previous study (8 (link)).