Western blotting was performed as described previously52 (link). Briefly, protein lysates were prepared in RIPA buffer supplemented with complete protease inhibitor (Roche Applied Science). Proteins were then separated on 6–15% SDS-PAGE, transferred onto PVDF membranes, and probed with anti-CIBZ [amino acids 1184–1197 (EQKDDIKAFAENVL) of CIBZ]17 (link), anti-Oct3/4 (MAB1759, R&D Systems), anti-Sox2 (S1451, Sigma-Aldrich), anti-Nanog (AB5731, Millipore), anti-α-tubulin (clone DM 1A, Sigma-Aldrich), anti-Brachyury (sc-17743, Santa Cruz Biotechnology), anti-Nkx-2.5 (sc-8697, Santa Cruz Biotechnology), anti-Islet 1 (ab109517, Abcam), anti-cTnI (ab19615, Abcam), and anti-MHC (clone MF20, Developmental Studies Hybridoma Bank) antibodies. HRP-conjugated anti-mouse or anti-rabbit IgG (Cell Signaling) were used as secondary antibodies.
Anti sox2 s1451
Manufactured by Merck Group
Anti-Sox2 (S1451) is a laboratory reagent used for research purposes. It is an antibody that specifically binds to the Sox2 protein, which is a transcription factor involved in the regulation of embryonic development and the maintenance of stem cell pluripotency. The core function of this product is to enable researchers to detect and study the expression and localization of the Sox2 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Complete protease inhibitor, by Roche (2 mentions)
Anti oct3 4 mab1759, by R&D Systems (2 mentions)
Anti nanog ab5731, by Merck Group (2 mentions)
Anti α tubulin clone dm1a, by Merck Group (2 mentions)
Hrp conjugated anti mouse or anti rabbit igg, by Cell Signaling Technology (1 mentions)
Ab109517, by Abcam (1 mentions)
Ab19615, by Abcam (1 mentions)
Anti nkx 2.5 sc 8697, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti sox2 s1451
1
Multiplex Western Blot Analysis
2
Protein Expression Analysis by Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blotting was performed as described previously (Sasai et al, 2005) . Briefly, protein lysates were prepared in RIPA buffer supplemented with complete protease inhibitor (Roche Applied Science). Proteins were then separated on 6%-15% SDS-PAGE, transferred onto PVDF membranes, and probed with anti-ZBTB38 (Oikawa et al, 2011) , anti-Oct3/4 (MAB1759, R&D Systems), anti-Sox2 (S1451, Sigma-Aldrich), anti-Nanog (AB5731, Millipore), anti-α-tubulin (clone DM 1A, Sigma-Aldrich) antibodies. HRP-conjugated anti-mouse or anti-rabbit IgG (Cell GelQuant.NET software provided by biochemlabsolutions.com.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!