Human kidney cancer cell lines 786-O and ACHN were purchased from Chinese Academy of Typical Culture Collection Cell Bank (Shanghai, China), cultured in 37 °C, 5% CO2, and saturated humidity. 786-O cells were maintained in RPMI 1640 (Gibco, Carlsbad, CA, USA) medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. ACHN cells grew in the same condition except in MEM (Gibco, Carlsbad, CA, USA) medium. Vismodegib was acquired from Medchem Express (NJ, USA). Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) were acquired from Peprotech (Rocky Hill, NJ, USA). Insulin and 2% B27 were acquired from Gibco. Primary antibodies including CD133, CD44, ALDHA1, Oct4, Nanog, PCNA, CyclinD1, Shh, Smo, Gli1, Gli2, and GAPDH were purchased from Proteintech (Rocky Hill, NJ, USA). Anti-rabbit and anti-mouse second antibodies were purchased from ZSGB-BIO (Beijing, China). PCR primers of CD133, CD44, ALDHA1, Oct4, and Nanog were synthesized by Beijing Genomics Institute (Beijing, China).
Aldha1
Manufactured by Proteintech
Sourced in United States
ALDHA1 is a recombinant protein used in research applications. It is a member of the aldehyde dehydrogenase family, which catalyzes the oxidation of aldehydes to carboxylic acids. The ALDHA1 protein is produced in a bacterial expression system and purified for use in various experiments.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions)
Proliferating cell nuclear antigen (pcna), by Proteintech (1 mentions)
Cd133, by Proteintech (1 mentions)
Nanog, by Proteintech (1 mentions)
Vismodegib, by MedChemExpress (1 mentions)
Gapdh, by Proteintech (1 mentions)
Cyclin d1, by Proteintech (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
Enhanced chemiluminescence kit, by Merck Group (1 mentions)
Proliferating cell nuclear antigen (pcna), by Abcam (1 mentions)
Bcl 2, by Abcam (1 mentions)
Gapdh, by Abcam (1 mentions)
Bcl2 associated x (bax), by Abcam (1 mentions)
Ab32503, by Abcam (1 mentions)
Glut1, by Abcam (1 mentions)
Ab15309, by Abcam (1 mentions)
Ab18197, by Abcam (1 mentions)
2 protocols using aldha1
1
Kidney Cancer Cell Proliferation Assay
2
Protein Isolation and Western Blot Analysis
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Total proteins were isolated using RIPA buffer and separated via 10% SDS-PAGE. These proteins were then transferred to PVDF membranes, which were blocked with 5% skimmed milk to prevent non-specific binding. Then, the membranes were incubated with the primary antibodies at 4 °C overnight and the secondary antibody for 1 h at room temperature. The antibodies were PCNA (ab18197; 1:1000; Abcam, Cambridge, UK), Bax (ab32503; 1:1000; Abcam), FABP4 (ab66682; 1:1000; Abcam), Bcl-2 (ab32124; 1:1000; Abcam), Sox2 (ab97959; 1:1000; Abcam), Oct4 (ab19857; 1:1000; Abcam), ALDHA1 (15910-1-AP; 1:1000; Proteintech, Wuhan, China), Glut1 (ab15309; 1:1000; Abcam), LDHA (ab52488; 1:1000; Ab-cam), p-ERK (4370; 1:1000; Cell Signaling Technology, Boston, MA, USA), ERK (4695; 1:1000; Cell Signaling Technology), p-mTOR (5536; 1:1000; Cell Signaling Technology), mTOR (2983; 1:1000; Cell Signaling Technology), and GAPDH (ab181602; 1:1000; Abcam). Finally, the enhanced chemiluminescence kit (Millipore, Billerica, MA, USA) was conducted to obtain the combined protein signals.
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