Plano convex lens

Manufactured by Thorlabs

A plano-convex lens is an optical element with one flat (planar) surface and one convex surface. The primary function of this lens is to refract light, allowing it to converge or diverge, depending on the curvature of the convex surface.

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Lab products found in correlation

10 mm pathlength quartz cuvette, by Starna Cells (1 mentions) Hr4000 spectrometer, by OceanOptics (1 mentions) N bk7 plano convex lens, by Thorlabs (1 mentions) Hnls008l, by Thorlabs (1 mentions) Zen software, by Zeiss (1 mentions)

3 protocols using plano convex lens

Upconverting Nanoparticle pH Cycling

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A CW
980 nm diode laser (Opto Engine) is fiber-coupled and focused onto
a 10 mm path length quartz cuvette (Starna Cells, Inc.) through a
collimator with an N-BK7 Plano convex lens (f = 20.0
mm) and an additional Plano convex lens (f = 35.0
mm) from Thorlabs. The incident irradiance is estimated to be 100
W/cm² with a power of 800 mW and beam diameter of 1 mm.
Emission is collected after a 750 nm SP filter by an OceanOptics HR4000
spectrometer.
For pH cycling, 0.48 M HCl and 0.48 M NaOH solutions
in DI water are prepared. pH is measured for a test sample containing
S-Medium buffer to calibrate the volume of acid or base necessary
to tune pH across the relevant range (pH 3 to pH 6) for five cycles.
On the basis of the calibration, HCl and NaOH are added dropwise to
the cuvette containing 1 mL of UCNPs in S-Medium (10 mg/mL). Spectra
is collected after shaking the cuvette using the setup mentioned above.
For intensity corrections, x is added to the raw
normalized intensity values. . This calculation assumes that intensity
and concentration are linearly related.

Lay A., Sheppard O.H., Siefe C., McLellan C.A., Mehlenbacher R.D., Fischer S., Goodman M.B, & Dionne J.A. (2019). Optically Robust and Biocompatible Mechanosensitive Upconverting Nanoparticles. ACS Central Science, 5(7), 1211-1222.

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Lens Refractive Index Measurements

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The refraction chamber was made from a clear acrylic box with a ¾” hole drilled in its side and a 1” disc of optical glass (Thorlabs: WG11050) glued to the outside. The mouse lenses were placed upon a pedestal made from a glass cloning cylinder and a piece of plastic glued to the top creating a small hole for the lens to rest on its equatorial side. A 0.8 mW, 632.8 nm helium/neon laser (Thorlabs: HNLS008L) was mounted on a vertically articulating platform and aimed through a Plano Convex Lens (Thorlabs: LA1131) which focused the beam parallel to the light axis of the mouse lens tissue. The chamber was flooded with phosphate-buffered saline (PBS) mixed with a small amount of powdered milk to increase the turbidity of the solution. Images of no fewer than 6 lenses dissected from at least 5 different animals and the surrounding media were taken following incremental raising (13–17 increments) of the platform-mounted laser such that the beam could be imaged across the equatorial plane of the lens. Measurements of focal lengths and the position of the beam within the lens were performed using Zen software (Zeiss). ANOVA analysis was used to determine whether statistical differences were observed between genotypes followed by post-hoc t-tests.

Martin J.B., Herman K., Houssin N.S., Rich W., Reilly M.A, & Plageman TF J.r. (2022). Arvcf Dependent Adherens Junction Stability is Required to Prevent Age-Related Cortical Cataracts. Frontiers in Cell and Developmental Biology, 10, 840129.

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Fluorescence Microscopy Protocol for Vm Dye Imaging

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Two red LEDs (each 7 W, center wavelength 660 nm; LED Engin, Wilmington, MA) were used as light sources for Vm dye excitation, which was driven from a stabilized current source (PLUMBUS [Pulsed LUMinos Bimodal Uniform Source]; donated from Aleksa Tech). LED light was collimated with a planoconvex lens (ThorLabs) and bandpass filtered with a 660/10 OD4 filter (Edmund Optics). Emitted florescence was filtered through a 700-nm long-pass OD6 filter (Chroma Optical) placed on the camera side. Fluorescence signals were acquired with an EMCCD camera (Evolve 128; Photometrics) at a resolution of 128 × 128 pixels, digitized at 16 bits at 500 frames per second, and transferred to a personal computer via real-time uninterrupted data transfer. A custom acquisition program was used for camera control.

Uzelac I., Iravanian S., Ashikaga H., Bhatia N.K., Herndon C., Kaboudian A., Gumbart J.C., Cherry E.M, & Fenton F.H. (2020). Fatal arrhythmias: Another reason why doctors remain cautious about chloroquine/hydroxychloroquine for treating COVID-19. Heart Rhythm, 17(9), 1445-1451.

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