Mak052 1 kt

Whole blood samples obtained from apo E −/− mice were centrifuged at 10,000 x g for 10 min at 4°C. After separation, the serum layer was carefully collected and used for further analysis. Commercially available biochemical assays were used to analyze serum concentrations of glucose (Abcam, ab65333), lactate dehydrogenase (Abcam, ab102526), alanine aminotransferase (Abcam, MAK052-1 KT), aspartate aminotransferase (Abcam, MAK055-1 KT), and cholesterol (Abcam, ab65390). 2–3 µl of serum was used for each analysis and quantified following manufacturer protocols. Glucose, lactate dehydrogenase, alanine aminotransferase groups were analyzed independently using One-Way ANOVA (sample distribution was normal for all groups) unmatched analysis with a single pooled variance for each data set, and a Tukey correction for pairwise comparisons only within the groups for individual data sets. The only exception was LDH levels, which were analyzed using an unpaired Kruskal-Wallis test with Dunn’s multiple comparisons test since the sample did not present a normal distribution.