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Tlr4 pe

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TLR4 PE is a fluorescently-labeled antibody that targets the Toll-like receptor 4 (TLR4) protein. TLR4 is a pattern recognition receptor that plays a role in the innate immune response.

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Lab products found in correlation

Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (2 mentions) Cellquest software, by BD (2 mentions) Ulbp3, by R&D Systems (2 mentions) Ulbp1 pe, by R&D Systems (2 mentions) Hla abc pe, by BD (2 mentions) Hla g fitc, by Bio-Rad (2 mentions) Mica micb fitc, by Bio-Rad (2 mentions) Cd155 fitc, by Bio-Rad (2 mentions) Ulbp 2 pe, by R&D Systems (2 mentions) Cd58 pe, by BD (2 mentions) Tlr9 fitc, by Abcam (2 mentions) Hla e, by Abcam (2 mentions) Cd112, by Bio-Rad (2 mentions) Cd26 pe, by BioLegend (1 mentions) 2 7 dichlorodihydrofluorescein diacetate dcfda, by Abcam (1 mentions) Mitotempo, by Merck Group (1 mentions)

3 protocols using tlr4 pe

1

Evaluating Surface Marker Expression in MSC Variants

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The expression of surface markers on MSC, nvMSC-HLA-G1, and vMSC-HLA-G1 was evaluated by flow cytometry using the following monoclonal anti-human antibodies: HLA-G FITC (ABD Serotec, Oxford, UK), HLA DR, DP, DQ FITC (BD Bioscience, San Jose, CA), HLA-ABC PE (BD Pharmingen, San Jose, CA), MICA/MICB FITC, CD155 FITC (both from ABD Serotec), ULBP-1 PE, ULBP-2 PE (both from R&D Systems), CD58 PE (BD Biosciences), TLR9 FITC, TLR4 PE (both from Abcam, Cambridge, UK) and TLR3 Alexa 647 (Imagenex, Port Coquitlam, BC, Canada). Cells were also stained for HLA E (Abcam), ULBP-3 (R&D Systems), and CD112 (ABD Serotec) using Alexa Fluor 488 Goat Anti-Mouse IgG (Molecular Probes-Life Technologies) as secondary antibody. Briefly, cells were incubated for 15 minutes in the dark with saturating concentrations of each antibody. Stained cells were then washed with phosphate-buffered saline 0.1% azide, fixed with 1% formaldehyde, and analyzed by flow cytometry using the CellQuest software (Becton Dickinson Biosciences, San Jose, CA). A total of 10,000 events was acquired for each sample. Appropriate isotype (negative) controls were performed for each antibody.
Boura J.S., Vance M., Yin W., Madeira C., Lobato da Silva C., Porada C.D, & Almeida-Porada G. (2014). Evaluation of gene delivery strategies to efficiently overexpress functional HLA-G on human bone marrow stromal cells. Molecular Therapy. Methods & Clinical Development, 1, 14041-.
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2

Characterizing Apoptosis and Inflammation in Lung Cells

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For apoptosis analysis, lung macrophages were stained with annexin V-FITC (fluorescein isothiocyanate) and propidium iodide (PI) (Imgenex Corporation) together with Vim-PE (phycoerythrin) (BD). ROS production was evaluated by staining cells with 2′,7′-dichlorodihydrofluorescein diacetate (DCFDA; Abcam) for 30 min and then cultured for additional 2 hours with CdCl2 and/or CB in the presence or absence of Mito-TEMPO (Sigma-Aldrich) before analysis by flow cytometry. Human fibroblasts were stained with CD26-PE (BioLegend), TLR1-FITC (Abcam), TLR2-FITC (Abcam), or TLR4-PE (Abcam) for measuring their surface expression in response to Vim or Cit-Vim (2 μg/ml) stimulation. Intracellular expression of α-SMA was detected by staining cells with α-SMA–APC (allophycocyanin) (R&D Systems), and TLR9 was characterized by staining unlabeled anti-TLR9 (Novus) followed by counterstaining with APC-conjugated anti-mouse immunoglobulin G (IgG). Data were acquired with LSR II (BD Biosciences) and analyzed using FlowJo software (version 10.6.1).
Li F.J., Surolia R., Li H., Wang Z., Liu G., Kulkarni T., Massicano A.V., Mobley J.A., Mondal S., de Andrade J.A., Coonrod S.A., Thompson P.R., Wille K., Lapi S.E., Athar M., Thannickal V.J., Carter A.B, & Antony V.B. (2021). Citrullinated vimentin mediates development and progression of lung fibrosis. Science translational medicine, 13(585), eaba2927.
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3

Evaluating Surface Marker Expression in MSC Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
The expression of surface markers on MSC, nvMSC-HLA-G1, and vMSC-HLA-G1 was evaluated by flow cytometry using the following monoclonal anti-human antibodies: HLA-G FITC (ABD Serotec, Oxford, UK), HLA DR, DP, DQ FITC (BD Bioscience, San Jose, CA), HLA-ABC PE (BD Pharmingen, San Jose, CA), MICA/MICB FITC, CD155 FITC (both from ABD Serotec), ULBP-1 PE, ULBP-2 PE (both from R&D Systems), CD58 PE (BD Biosciences), TLR9 FITC, TLR4 PE (both from Abcam, Cambridge, UK) and TLR3 Alexa 647 (Imagenex, Port Coquitlam, BC, Canada). Cells were also stained for HLA E (Abcam), ULBP-3 (R&D Systems), and CD112 (ABD Serotec) using Alexa Fluor 488 Goat Anti-Mouse IgG (Molecular Probes-Life Technologies) as secondary antibody. Briefly, cells were incubated for 15 minutes in the dark with saturating concentrations of each antibody. Stained cells were then washed with phosphate-buffered saline 0.1% azide, fixed with 1% formaldehyde, and analyzed by flow cytometry using the CellQuest software (Becton Dickinson Biosciences, San Jose, CA). A total of 10,000 events was acquired for each sample. Appropriate isotype (negative) controls were performed for each antibody.
Boura J.S., Vance M., Yin W., Madeira C., Lobato da Silva C., Porada C.D, & Almeida-Porada G. (2014). Evaluation of gene delivery strategies to efficiently overexpress functional HLA-G on human bone marrow stromal cells. Molecular Therapy. Methods & Clinical Development, 1, 14041-.
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