Protein extraction and western blot analyses were performed as described previously [19 (link)]. Bax (sc-526), Bcl-2 (sc-492), ATG12 C6 (sc-271688), and GAPDH (sc-32233) antibodies and a mouse anti-goat IgG-HRP secondary antibody (sc-2354) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), NIX from Sigma-Aldrich (Madrid, Spain), and HRP goat anti-mouse IgG from BD Pharmingen™ (San Jose, CA, USA). The proteins were visualized using a Western Lightning Plus-ECL chemiluminescence kit (PerkinElmer, Billerica, MA, USA) according to the manufacturer's protocol. Images were analyzed using the Kodak Image Station 2000R (Eastman Kodak Company, Rochester, NY, USA). Protein band intensity was normalized to GAPDH, and data were expressed in percentage terms relative to controls.
Mouse anti goat igg hrp secondary antibody sc 2354
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Mouse anti-goat IgG-HRP secondary antibody (sc-2354) is a laboratory reagent used in immunodetection techniques. It is a secondary antibody that binds to goat primary antibodies and is conjugated with horseradish peroxidase (HRP) enzyme for signal amplification.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh sc 32233, by Santa Cruz Biotechnology (1 mentions)
Hrp goat anti mouse igg, by BD (1 mentions)
Western lightning plus ecl chemiluminescence kit, by PerkinElmer (1 mentions)
Image station 2000r, by Kodak (1 mentions)
Horse anti mouse 7076, by Cell Signaling Technology (1 mentions)
Goat anti rabbit 7074, by Cell Signaling Technology (1 mentions)
2 protocols using mouse anti goat igg hrp secondary antibody sc 2354
1
Western Blot Analysis of Apoptosis Regulators
2
Pharmacological Modulation of hERG Channels
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Bisindolylmaleimide I (BIM-1, ab144264) was purchased from Abcam (Cambridge, MA). Gö 6983 (CAS no. 133053-19-7) and Sotrastaurin (STN, also known as AEB071, CAS no. 425637-18-9) were purchased from Cayman Chemical Company (Ann Arbor, MI). Goat anti-hERG (C20, sc-15968, C-terminal), mouse anti-hERG (F-12, sc-377388, N-terminal), and goat anti-integrin b1 (sc-6622) primary antibodies and mouse anti-goat IgG-HRP secondary antibody (sc-2354) were purchased from Santa Cruz Biotechnology (Dallas, TX). Mouse anti-hERG (DT-331, S5-Pore linker) antibody was purchased from D.I.V.A.L. (Toscana S.R.L., Florence, Italy). Mouse anti-actin primary antibody (A4700) was purchased from Sigma-Aldrich. Rabbit antiphosphorylated Nedd4-2 (p-Nedd4-2, Ser-448, 8063S) primary antibody, horse anti-mouse (7076S), and goat anti-rabbit (7074S) secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA). The PKC sampler kit containing isoenzyme specific antibodies (611421) was obtained from BD Biosciences (Franklin Lakes, NJ). Electrolytes and chemicals for patch-clamp recordings were purchased from Sigma-Aldrich.
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