The cell apoptosis was determined with Hoechst 33258 after EC-1 and ECa-109 cells were treated with 0.6 and 1.3 μg/mL Tanshinone IIA for 48 h, respectively. After 48 h, cells were washed with 1 × PBS three times and stained with 1 μg/mL of Hoechst 33258 nuclear dye (Beyotime, China) for 30 min at 37°C. Images were obtained by fluorescence microscopy (Nikon Ti-S, Japan).
Hoechst 33258 nuclear dye
Manufactured by Beyotime
Sourced in China
Hoechst 33258 is a fluorescent dye used in various laboratory applications. It binds selectively to the minor groove of DNA, allowing for the visualization and quantification of nucleic acids. The core function of Hoechst 33258 is to provide a reliable and sensitive method for detecting and analyzing DNA in biological samples.
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Lab products found in correlation
2 protocols using hoechst 33258 nuclear dye
1
Tanshinone IIA Induces Apoptosis
2
Quantifying Apoptosis via Hoechst Staining
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The cell apoptosis was determined with Hoechst 33258 staining. HT-29 cells were treated with 20, 40, and 80 µM UA. After 24 h, cells were washed three times with phosphate buffered saline (PBS) and stained with 1 µg/mL of Hoechst 33258 nuclear dye (Beyotime, China) for 30 min at 37°C. Cells were imaged under fluorescence microscopy (Nikon Ti-S, Japan) to assess chromatin condensation and segregation.
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