Ctgf l 20

Manufactured by Santa Cruz Biotechnology

Sourced in United States

CTGF (L-20) is a primary antibody product offered by Santa Cruz Biotechnology. It is a polyclonal antibody raised against a peptide mapping at the N-terminus of CTGF of human origin. The antibody is intended for the detection of CTGF by various biochemical techniques.

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Lab products found in correlation

β catenin d10a8, by Cell Signaling Technology (1 mentions) Lmnb1, by Cell Signaling Technology (1 mentions) Yap d8h1x, by Cell Signaling Technology (1 mentions) Tiam1, by Fortis Life Sciences (1 mentions) Smad7 b 8, by Santa Cruz Biotechnology (1 mentions) Tgf β, by Merck Group (1 mentions) Gapdh, by GeneTex (1 mentions) Rat tail collagen 1, by Corning (1 mentions) D luciferin, by GoldBio (1 mentions) Hpa008572, by Merck Group (1 mentions) Phosphotyrosine p tyr 100, by Cell Signaling Technology (1 mentions) Actin ac15, by Merck Group (1 mentions) β catenin, by BD (1 mentions) Imatinib, by Cayman Chemical (1 mentions) C3 transferase, by Cytoskeleton (1 mentions) Y 27632, by Enzo Life Sciences (1 mentions)

3 protocols using ctgf l 20

Western Blot Analysis of Signaling Proteins

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Protein extracts were resolved by SDS-PAGE and transferred to nylon membranes (ImmobilonP) using standard techniques. Primary antibodies used for Western blotting were as follows: TIAM1 (1:1000; Bethyl, A300-099A), TIAM1 (1:1000; R&D Systems, AF5038), TAZ (1:200; BD Biosciences, 560235), TAZ (1:500; Sigma-Aldrich, HPA0074), YAP (D8H1X; 1:1000; Cell Signaling Technology, 14074), LMNB1(1:1000; Cell Signaling Technology, 12586), βTrCP (D13F10; 1:1000; Cell Signaling Technology, 4394), βTrCP (1B1D2; 1:50; Invitrogen, 37-3400), AXIN (C76H11; 1:1000; Cell Signaling Technology, 2087), β-catenin (D10A8; 1:1000; Cell Signaling Technology, 8480), TEAD4 (1:1000; Abcam, ab58310), panTEAD (D3F7L; 1:1000; Cell Signaling Technology, 13295), CTGF (L-20; 1:200; SantaCruz Biotechnology, sc14939) and α-tubulin (DM1A; 1:2000; Sigma-Aldrich, T9026).

Diamantopoulou Z., White G., Fadlullah M.Z., Dreger M., Pickering K., Maltas J., Ashton G., MacLeod R., Baillie G.S., Kouskoff V., Lacaud G., Murray G.I., Sansom O.J., Hurlstone A.F, & Malliri A. (2017). TIAM1 Antagonizes TAZ/YAP Both in the Destruction Complex in the Cytoplasm and in the Nucleus to Inhibit Invasion of Intestinal Epithelial Cells. Cancer Cell, 31(5), 621-634.e6.

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Comprehensive Molecular Profiling Assay

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TGFβ and antibodies against fibronectin-1 and α-SMA were from Sigma-Aldrich,Taufkirchen, Germany; SLM60314343 was purchased from Tocris (Bio-Techne GmbH, Wiesbaden, Germany); HWG-35D was synthesized and characterized as previously described 16 (link) ; the Collagen1 antibody was from Merck Millipore, Darmstadt, Germany; GAPDH was from GeneTex, Irvine, CA, USA; CTGF (L-20) and Smad7 (B-8) antibodies were from Santa Cruz Biotechnology, Heidelberg, Germany; all cell culture nutrients were from Life Technologies, Karlsruhe, Germany.

Schwalm S., Beyer S., Hafizi R., Trautmann S., Geisslinger G., Adams D.R., Pyne S., Pyne N., Schaefer L., Huwiler A, & Pfeilschifter J. (2021). Validation of highly selective sphingosine kinase 2 inhibitors SLM6031434 and HWG-35D as effective anti-fibrotic treatment options in a mouse model of tubulointerstitial fibrosis. Cellular signalling, 79.

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Integrin-Mediated Cytoskeletal Regulation

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Antibodies used were: α3 integrin, A3-X8 (8 (link)); A3-CYT (15 (link)); and HPA008572, (Sigma-Aldrich); RhoA (ARHO1, Cytoskeleton Inc);
RhoC, (D40E4, Cell Signaling Technology); actin (AC-15, Sigma-Aldrich); LATS1
C66B5, YAP/TAZ D24E4 (Cell Signaling Technology); TAZ/WWTR1 (1H9, Lifespan
Biosciences, Inc); YAP1 (H-125, Santa Cruz Biotechnology); CTGF (L-20, Santa
Cruz Biotechnology); β-catenin (610153, BD Biosciences); p190RhoGAP
(D2D6, Millipore); phosphotyrosine (P-Tyr-100, Cell Signaling Technology);
p120RasGAP (B4F8, Santa Cruz Biotechnology); Crk (22/Crk, BD Biosciences);
phospho-Crk/CrkL, (#3181, Cell Signaling Technology); Arg/Abl2
(A301-986A, Bethyl Laboratories, Inc); andFAK and phospho-FAK (77/FAK and 18/FAK
(pY397), BD Biosciences). Secondary reagents were PE-goat anti-mouse, Alexa 680
goat anti-mouse, Alexa 790 goat anti-rabbit, and Dylight 800 Neutravidin (Thermo
Fisher Scientific).
Other reagents were D-luciferin (Gold Biotechnology), rat tail collagen I
(Corning), C3 transferase (Cytoskeleton, Inc), lysophasphatidic acid (LPA)
(Avanti Polar Lipids), Y-27632 (Enzo Life Sciences), blebbistain (Selleckchem),
imatinib (Cayman Chemical), DPH
[5-(1,3-diaryl-1H-pyrazol-4-yl)hydantoin,
5-[3-(4-fluorophenyl)-1-phenyl-1H-pyrazol-4-yl]-2,4-imidazolidinedione]
(Sigma-Aldrich), and PF-573228 (Tocris).

Varzavand A., Hacker W., Ma D., Gibson-Corley K., Hawayek M., Tayh O.J., Brown J.A., Henry M.D, & Stipp C.S. (2016). α3β1 Integrin suppresses prostate cancer metastasis via regulation of the Hippo pathway. Cancer research, 76(22), 6577-6587.

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