Single cell suspensions were obtained from MLNs, spleens, peritoneal lavage, and peripheral blood after bone marrow reconstitution and N. brasiliensis infection. Cells were blocked with unlabeled anti-mouse CD16/32 (clone 2.4G2) for 10min on ice, followed by incubation with fluorochrome labeled antibodies. Antibodies used were as follows Alexa647 anti-mouse CD45.1 (clone A20), PerCP-Cy5.5 anti-mouse CD45.2 (clone 104) (Biolegend), FITC lineage cocktail, CD127 (clone A7R34), ST2 (clone DIH9), CD117 (clone 2B8), and Fcεr1α (clone MAR-1) (Biolegend). Cells were analyzed on a BD Canto Flow analyzer; data was analyzed using FlowJo software (version7.6, TreeStar, Ashland, OR). For FACS, single cell suspensions of MLNs were stained with PE anti-mouse CD90.2 (clone 30.H12), APC anti-mouse/human B220 (clone RA3-6B2), PE-Cy7 anti-mouse CD4 (clone GK1.5), and FITC anti-mouse CD11c (clone N418) (Biolegend) and then sorted on a BD FACS Aria cell sorter (BD Biosciences, San Jose, CA).
Alexa647 anti mouse cd45.1 clone a20
Manufactured by BioLegend
Alexa647 anti-mouse CD45.1 (clone A20) is a fluorescently-labeled antibody that binds to the mouse CD45.1 protein. CD45.1 is a commonly used allelic variant of the CD45 protein, which is expressed on the surface of most hematopoietic cells.
Automatically generated - may contain errors
Lab products found in correlation
Percp cy5.5 anti mouse cd45 2 clone 104, by BioLegend (2 mentions)
Fcεr1α clone mar 1, by BioLegend (2 mentions)
Canto flow analyzer, by BD (2 mentions)
Pe cy7 anti mouse cd4 clone gk1.5, by BioLegend (2 mentions)
Fitc anti mouse cd11c clone n418, by BioLegend (2 mentions)
Facsaria cell sorter, by BD (2 mentions)
2 protocols using alexa647 anti mouse cd45.1 clone a20
1
Phenotypic Characterization of Immune Cells
2
Phenotypic Characterization of Immune Cells
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Single cell suspensions were obtained from MLNs, spleens, peritoneal lavage, and peripheral blood after bone marrow reconstitution and N. brasiliensis infection. Cells were blocked with unlabeled anti-mouse CD16/32 (clone 2.4G2) for 10min on ice, followed by incubation with fluorochrome labeled antibodies. Antibodies used were as follows Alexa647 anti-mouse CD45.1 (clone A20), PerCP-Cy5.5 anti-mouse CD45.2 (clone 104) (Biolegend), FITC lineage cocktail, CD127 (clone A7R34), ST2 (clone DIH9), CD117 (clone 2B8), and Fcεr1α (clone MAR-1) (Biolegend). Cells were analyzed on a BD Canto Flow analyzer; data was analyzed using FlowJo software (version7.6, TreeStar, Ashland, OR). For FACS, single cell suspensions of MLNs were stained with PE anti-mouse CD90.2 (clone 30.H12), APC anti-mouse/human B220 (clone RA3-6B2), PE-Cy7 anti-mouse CD4 (clone GK1.5), and FITC anti-mouse CD11c (clone N418) (Biolegend) and then sorted on a BD FACS Aria cell sorter (BD Biosciences, San Jose, CA).
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