Tissues were fixed in 10% formalin overnight and embedded in paraffin. PDAC sections were deparaffinized in xylene, rehydrated through sequential ethanol, and rinsed in PBS. Non-specific signals were blocked using 10% goat serum in 0.1% Triton X-100. Tumor samples were stained with the following primary antibodies: αSMA(Sigma, A5228, 1:500), BCAT1 (Sigma, HPA048592, 1:200), SMAD5 (Sigma, HPA058931, 1:200), Ki-67(Santa Cruz Biotechnology,sc-239001:500), PCNA(Santa Cruz Biotechnology, sc-561:500). After overnight incubation, the slides were washed and incubated with biotinylated secondary antibody (Vector Laboratories) for 30min at room temperature. All slides were then incubated with avidin-biotin peroxidase complex for 30 min, and the signals were visualized by using DAB Substrate Kit (Vector Laboratories). The tissue sections were counterstained with VECTOR Hematoxylin QS and mounted with VectaMount after dehydration. IF staining was performed on tissue slices or chamber slide cultures (Thermo Fisher Scientific). The primary antibody was Anti-Proteasome 20S alpha + beta (Abcam, ab22673). Samples were mounted on microscope slides with Prolong Antifade with DAPI and imaged using a Nikon A1Si Laser Scanning Confocal Microscope.
Chamber slide cultures
Manufactured by Thermo Fisher Scientific
Sourced in United States
Chamber slide cultures are laboratory equipment designed for cell culture applications. They provide a contained environment for culturing cells and tissues, allowing for controlled growth conditions and easy observation.
Automatically generated - may contain errors
Lab products found in correlation
Biotinylated secondary antibody, by Vector Laboratories (2 mentions)
α sma, by Merck Group (2 mentions)
A1si laser scanning confocal microscope, by Nikon (2 mentions)
Dab substrate kit, by Vector Laboratories (2 mentions)
Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (2 mentions)
Vector hematoxylin qs, by Vector Laboratories (2 mentions)
Vectamount, by Vector Laboratories (2 mentions)
Bcat1, by Merck Group (2 mentions)
Smad5, by Merck Group (2 mentions)
Anti proteasome 20s alpha beta, by Abcam (2 mentions)
Anti γh2ax antibody, by Cell Signaling Technology (1 mentions)
Axion vision rel 4, by Zeiss (1 mentions)
Anti ddup antibody, by Sino Biological (1 mentions)
Anti pcna, by Cell Signaling Technology (1 mentions)
3 protocols using chamber slide cultures
1
Immunohistochemistry and Immunofluorescence Analysis of Pancreatic Ductal Adenocarcinoma
2
Antibody Imaging Analysis in Cell Cultures
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The indicated cells were plated on chamber slide cultures (Thermo Fisher Scientific, CA, USA), and then treated with anti-DDUP antibody (Sino Biological, Wuhan, China), anti-γH2AX antibody (#9718, Cell Signaling Technology, MA, USA), anti-PCNA (#2586, Cell Signaling Technology, MA, USA), anti-RAD18 antibody (#9040, Cell Signaling Technology, MA, USA), or anti-RAD51C antibody (PA5-77078, Invitrogen, USA). The photographs were taken with the Axion Vision Rel.4.6 computerized image analysis system (Carl Zeiss, Jena, Germany).
3
Immunohistochemistry and Immunofluorescence Analysis of Pancreatic Ductal Adenocarcinoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissues were fixed in 10% formalin overnight and embedded in paraffin. PDAC sections were deparaffinized in xylene, rehydrated through sequential ethanol, and rinsed in PBS. Non-specific signals were blocked using 10% goat serum in 0.1% Triton X-100. Tumor samples were stained with the following primary antibodies: αSMA(Sigma, A5228, 1:500), BCAT1 (Sigma, HPA048592, 1:200), SMAD5 (Sigma, HPA058931, 1:200), Ki-67(Santa Cruz Biotechnology,sc-239001:500), PCNA(Santa Cruz Biotechnology, sc-561:500). After overnight incubation, the slides were washed and incubated with biotinylated secondary antibody (Vector Laboratories) for 30min at room temperature. All slides were then incubated with avidin-biotin peroxidase complex for 30 min, and the signals were visualized by using DAB Substrate Kit (Vector Laboratories). The tissue sections were counterstained with VECTOR Hematoxylin QS and mounted with VectaMount after dehydration. IF staining was performed on tissue slices or chamber slide cultures (Thermo Fisher Scientific). The primary antibody was Anti-Proteasome 20S alpha + beta (Abcam, ab22673). Samples were mounted on microscope slides with Prolong Antifade with DAPI and imaged using a Nikon A1Si Laser Scanning Confocal Microscope.
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