Hrp conjugated donkey anti rat igg

Manufactured by Jackson ImmunoResearch

HRP-conjugated donkey anti-rat IgG is a secondary antibody that binds to rat immunoglobulin G (IgG) and is conjugated to horseradish peroxidase (HRP). It is designed for use in immunoassays and other applications where the detection of rat IgG is required.

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Lab products found in correlation

Alexafluor 647 conjugated goat anti rat igg, by BioLegend (2 mentions) Mouse anti rat igg2a, by BioXCell (2 mentions) Alexafluor 647 conjugated mouse anti human igg, by BioLegend (2 mentions) Alexafluor 647 conjugated rat anti mouse cd45, by BioLegend (2 mentions) Bv650 conjugated rat anti mouse human cd11b, by BioLegend (2 mentions) Pe cy5 conjugated rat anti mouse cd19, by BioLegend (2 mentions) Pe conjugated donkey anti mouse igg, by Jackson ImmunoResearch (2 mentions) Hrp conjugated donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Mouse anti rpe65, by Novus Biologicals (1 mentions) Anti sod1, by Proteintech (1 mentions) Hrp conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Rabbit anti calreticulin, by Cell Signaling Technology (1 mentions) Anti catalase, by Proteintech (1 mentions) Rabbit anti syntenin 1, by Abcam (1 mentions) Hoechst 33258, by Thermo Fisher Scientific (1 mentions) Triton x 100, by Merck Group (1 mentions) 3 3 5 5 tetramethylbenzidine (tmb), by BD (1 mentions) Streptavidin hrp, by Agilent Technologies (1 mentions) Blocking buffer, by Agilent Technologies (1 mentions) Peroxidase substrate kit, by Vector Laboratories (1 mentions)

Close spelling variants of this product

Donkey anti-rat HRP Anti-rat-HRP

6 protocols using hrp conjugated donkey anti rat igg

Multicolor Flow Cytometry Panel for Mouse Immune Cell Analysis

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Rat anti-mouse α₄β₇ integrin (Clone DATK32, Biolegend)
Rat anti-mouse β₇ integrin (Clone FIB504, BioXCell)
Mouse anti-rat IgG_2a (Clone RG7/1.30, BioXCell)
Rat IgG_2a isotype control (Clone 2A3, BioXCell)
PE-conjugated donkey anti-mouse IgG (Polyclonal, Jackson Immuno Research)
AlexaFluor 647-conjugated goat anti-rat IgG (Polyclonal, Biolegend)
AlexaFluor 647-conjugated mouse anti-human IgG (Polyclonal, Biolegend)
AlexaFluor 647-conjugated rat anti-mouse CD45 (Clone 30-F11, Biolegend)
AlexaFluor 488-conjugated rat anti-mouse CD45 (Clone 30-F11, Biolegend)
BV650-conjugated rat anti-mouse/human CD11b (Clone M1/70, Biolegend)
PE/Cy5-conjugated rat anti-mouse CD19 (Clone 6D5, Biolegend)
BV421-conjugated hamster anti-mouse CD3ε (Clone 145-2C11, Biolegend)
APC/Cy7-conjugated rat anti-mouse CD4 (Clone GK1.5, Biolegend)
AlexaFluor 488-conjugated rat anti-mouse CD8a (Clone 53-6.7, Biolegend)
PE-conjugated rat anti-mouse CD8a (Clone 53-6.7, Biolegend)
PE/Dazzle 594 rat anti-mouse CD25 (Clone PC61, Biolegend)
BV510 armenian hamster anti-mouse CD69 (Clone H1.2F3, Biolegend)
PE-conjugated rat anti-mouse FOXP3 (Clone MF-14, Biolegend)
HRP-conjugated donkey anti-rat IgG (Polyclonal, Jackson Immuno Research)

Dammes N., Goldsmith M., Ramishetti S., Dearling J.L., Veiga N., Packard A.B, & Peer D. (2021). Conformation Sensitive Targeting of Lipid Nanoparticles for RNA Therapeutics. Nature nanotechnology, 16(9), 1030-1038.

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Antibody Characterization in Cell Research

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Calcium and magnesium free PBS (PBS; #10010‐023), and Hoechst 33258 (#H3569) were from Invitrogen (Waltham, MA). Triton X‐100 (#T8787) was obtained from Sigma–Aldrich (St. Louis, MO). Antibodies used in the current study were as follows: Mouse anti‐RPE65 (#NB100‐355) [clone 401.8B11.3D9]; Novus Biologicals, Centennial, CO), mouse anti‐DSG1 (#610273, BD Transduction, Franklin Lakes, NJ), mouse anti‐Cytokeratin 10 (#MA5‐13705 [clone DE‐K10]; ThermoFisher, Waltham, MA), rabbit anti‐Syntenin‐1 (#ab19903]; Abcam, Cambridge, MA), mouse anti‐Annexin II (#610068, BD Transduction Laboratories, Franklin Lakes, NJ), rat anti‐Integrin Beta 1 (AIIB2 clone, Developmental Studies Hybridoma Bank, Iowa City, IA), mouse anti‐Occludin (#66378‐1‐Ig, Proteintech, Rosemont, IL), rabbit anti‐Calreticulin (#12238 [clone D3E6]; Cell Signaling Technologies, Danvers, MA), anti‐SOD1 (#10269‐1‐AP, Proteintech), anti‐Catalase (#66765‐1‐Ig, Proteintech), anti‐MDA (#NBP2‐59367, Novus Biologicals), anti‐HSPA5 (#PA5‐19503, ThermoFisher), anti‐CLDN19 (#SC‐36597, Santa Cruz Technology, Dallas, TX), HRP‐conjugated donkey‐anti‐rat IgG (#712‐035‐153, Jackson ImmunoResearch Laboratories, West Grove, PA), HRP‐conjugated donkey‐anti‐mouse IgG (#715‐035‐150, Jackson ImmunoResearch) and HRP‐conjugated donkey‐anti‐rabbit IgG (#711‐035‐152, Jackson ImmunoResearch).

Hernandez B.J., Skiba N.P., Plössl K., Strain M., Liu Y., Grigsby D., Kelly U., Cady M.A., Manocha V., Maminishkis A., Watkins T., Miller S.S., Ashley‐Koch A., Stamer W.D., Weber B.H., Bowes Rickman C, & Klingeborn M. (2023). Polarized desmosome and hemidesmosome shedding via small extracellular vesicles is an early indicator of outer blood‐retina barrier dysfunction. Journal of Extracellular Biology, 2(10), e116.

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Indirect ELISA for GAA Antibody Detection

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An indirect ELISA was used to detect GAA specific antibodies. Plates were coated with rGAA (Myozyme, Genzyme Corporation) overnight, rinsed, and blocked with 5% milk in PBS. Samples in serial dilutions, controls, and blanks were added and incubated for 2 h at 37 °C. HRP conjugated donkey anti-rat IgG (1:5000, r712–035-150; Jackson Immuno Research) followed by Streptavidin/HRP (1:1000, P 0397; DakoCytomation) was added and 3,3′,5,5′- Tetramethylbenzidine (TMB, BD Biosciences) was used as substrate. Reactions were stopped with 2 N H2SO4 and reading was determined at 450 nm. Positivity was determined with control sera collected prior to the injections using a cut-off value of 0.200 OD.

Hordeaux J., Dubreil L., Robveille C., Deniaud J., Pascal Q., Dequéant B., Pailloux J., Lagalice L., Ledevin M., Babarit C., Costiou P., Jamme F., Fusellier M., Mallem Y., Ciron C., Huchet C., Caillaud C, & Colle M.A. (2017). Long-term neurologic and cardiac correction by intrathecal gene therapy in Pompe disease. Acta Neuropathologica Communications, 5, 66.

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Immunohistochemical Analysis of Germinal Centers

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To examine GC architecture, spleens were removed from mice and fixed in 10% formalin (Sigma-Aldrich). For immunostaining, paraffin sections where deparaffined, hydrated and treated with antigen retrieval buffer (Dako). The sections then were permeabilized by 4 min treatment with Triton X-100 (0.1% in PBS) and incubated for 90 min with blocking buffer (Dako) followed by an overnight incubation with primary antibodies: rat anti-mouse B220 and hamster anti-mouse CD3e (1/1000, BD Pharmingen). For immunohistochemistry, antibodies were detected with AP-conjugated goat anti-Armenian hamster IgG or HRP-conjugated donkey anti-rat IgG (1/10000, Jackson ImmunoResearch Laboratories). HRP was reacted with DAB (Peroxidase Substrate Kit; Vector), and alkaline phosphatase with Fast Blue/Napthol AS-MX (Sigma-Aldrich). Levamisole (Sigma) was used to block endogenous alkaline phosphatase activity and slides were mounted in Crystal Mount (Electron Microscopy Sciences). Sections were viewed under a Nikon Microphot FXA light microscope and photographs were taken with a Spot Insight Camera, using 10× and 40× objectives, then analyzed using Spot Advanced software (Diagnostic Instruments) and ImageJ (NIH).

Baeza Garcia A., Siu E., Sun T., Exler V., Brito L., Hekele A., Otten G., Augustijn K., Janse C.J., Ulmer J.B., Bernhagen J., Fikrig E., Geall A, & Bucala R. (2018). Neutralization of the Plasmodium-encoded MIF ortholog confers protective immunity against malaria infection. Nature Communications, 9, 2714.

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Immunophenotyping of Mouse Colonic Tissues

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Mouse colonic tissues were harvested, swiss-rolled, and fixed with 4% paraformaldehyde (Sigma, St. Louis, MO) overnight. Fixed tissues were then embedded in OCT compound (Sakura, Tokyo, Japan) and kept at −80°C. Frozen tissue sections (7 μm) were first blocked for 30 min at room temperature with Blocking Reagent (PerkinElmer, Waltham, MA) and then incubated overnight at 4°C with primary antibodies as described below. Following overnight incubation, sections were washed and then incubated with HRP-conjugated secondary antibody for 1h at room temperature. After washing, sections were treated with tyramide-fluorescein (PerkinElmer), followed by counterstaining with DAPI (Sigma). All stained sections were analyzed by confocal microscope using either the 10× or the 20× objective (TCS SP2, Leica, Wetzlar, Germany). Images were processed with Adobe Photoshop software. The following antibodies were used: anti-CD11c and anti-F4/80 from BD Bioscience (Franklin Lakes, NJ), anti-CD3, anti-NKp46, and anti-B220 from eBioscience (San Diego, CA), HRP-conjugated donkey anti-rat IgG, and HRP-conjugated donkey anti-Armenian hamster IgG (H+L) from Jackson Immunoresearch (West Grove, PA).

Kobayashi T., Steinbach E.C., Russo S.M., Matsuoka K., Nochi T., Maharshak N., Borst L.B., Hostager B., Garcia-Martinez J.V., Rothman P.B., Kashiwada M., Sheikh S.Z., Murray P.J, & Plevy S.E. (2014). NFIL3 deficient mice develop microbiota dependent, IL-12/23 driven spontaneous colitis. Journal of immunology (Baltimore, Md. : 1950), 192(4), 1918-1927.

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Multicolor Flow Cytometry Panel for Mouse Immune Cell Analysis

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