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Dr1010

Manufactured by HACH
Sourced in United States

The DR1010 is a portable spectrophotometer designed for water analysis. It provides accurate measurements of various water quality parameters.

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5 protocols using dr1010

1

Comprehensive Water Quality Analysis

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The chemical oxygen demand (COD) was determined using a COD colorimeter (DR1010, HACH, Loveland, CO, USA) with a digestion device (DRB200, HACH) according to the US EPA approved HACH Method No. 8000. The pH value was measured using a pH meter (pHS-3C, Leici, INESA Scientific Instrument Co., Ltd., Shanghai, China) with Glass electrode method (Chinese National Standard GB 6920-86). NH3-N and TP were determined using an ultraviolet/visible spectrophotometer (T6xinyue, HuaBi Scientific Instrument Co., Ltd. Nanjing, China) with Nascar reagent photometry and Chinese National Standard GB 11893-89. PVA was analyzed using the UV–Vis spectrophotometer (DR 6000, HACH) at 690 nm, based on the blue color produced by the reaction of PVA with iodine (analytically pure, Tianjin Zhiyuan Chemical Reagent Co. Ltd., Tianjin, China) in the presence of boric acid (analytically pure, Tianjin Zhiyuan Chemical Reagent Co. Ltd.) [2 (link)]. The turbidity of the PML was determined using a turbidity meter (ET93810, Lovibond® Tintometer Group, Dortmund, Germany) according to the US EPA 180.1 method. The BOD5 was determined according to the pressure sensor method with an automated measurement apparatus. The measurement of BOD (20 °C, 5 days) was performed using a pressure sensor method with an automated measurement apparatus (BOD Trak II, HACH).
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2

Analytical Techniques for Wastewater Treatment

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Samples collected from the inlet and outlet of the two reactors was filtered through 0.45 μm membrane before analysis. NO3−-N was determined by UV-spectrophotometer (Shimadzu UVmini-1240, Kyoto, Japan) at 220 and 275 nm. Total nitrogen (TN) was measured by oxidizing all the nitrogen components to nitrate nitrogen using alkaline potassium persulfate. NO2−-N was detected according to naphthylethylenediamine hydrochloride spectrophotometry method with visible spectrophotometer (Spectrum SP-721E, Shenzhen, China). CODCr concentration of the influent and effluent were determined by Portable COD Tester (Hach DR1010, Loveland, USA). Fluorescence EEM measurements were conducted by using a fluorescence spectrophotometer (Hitachi F-700, Tokyo, Japan). The biomass contents on the surface of the two carriers were quantified according to Jin et al. [27 (link)] The methods used for PCR-DGGE analysis were the same as those described previously by Dong et al. [28 (link)].
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3

Comprehensive Characterization of Activated Sludge

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The total organic carbon (TOC) of LB-EPS and TB-EPS extracts was detected using a TOC analyzer (multi N/C 2100 S, Analytik Jena AG, Jena, Germany). The DO concentration in mixed liquid was examined with a DO analyzer (Pro20, YSI, Columbus, Ohio, USA). The COD in effluent was detected using a COD instrument (DR1010, HACH, Loveland, Colorado, USA). The SVI, suspended solids (SS), volatile suspended solids (VSS), and TP in the bulk solution (TPsolution), LB-EPS (TPLB-EPS), and TB-EPS (TPTB-EPS) were measured according to standard methods [28 ]. The TP in sludge (TPSludge) was measured after resuspended sludge was dispersed by sonication, and the TP in microbial cells (TPcell) was calculated by subtraction. The metal elements in the bulk solution, sludge, LB-EPS, and microbial cells were directly measured by ICP-OES (Agilent 715, Agilent Technologies Inc., Palo Alto, CA, USA). The metal elements in TB-EPS were calculated by subtraction. The morphology of microorganisms in the activated sludge was observed by a scanning electron microscope (MIRA 3 GMU/GMH; TESCAN, Brno, Czechoslovakia).
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4

COD Determination in PML Samples

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A 2 mL PML sample was placed in a HACH special digestion colorimeter tube (160 mm × 100 mm) and mixed well with a digestion solution for COD (Cat.2125916, Pk/150, Range 20–1500 mg·L−1, HACH) and digested at 150 °C for 2 h in a Trak II digestion apparatus (DRB200, HACH). After cooling to room temperature, the vial was transferred into the COD colorimeter (DR1010, HACH) for COD determination.
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5

Fenton Oxidation of Aniline Using Catalysts

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The Fenton oxidation of aniline was performed in a 250 mL flask equipped with a water bath thermostat as the reactor. In a typical oxidation experiment, a 200 mL aniline aqueous solution (500 mg L−1) was added to the reactor. The temperature of the reaction system was heated to 50 °C by a water bath, and then a 2.0 mL H2O2 solution (30 wt%) was added to the above aniline solution (thus the concentration of H2O2 being 0.087 mol L−1 at the beginning of the reaction). At the absence of a catalyst, it was confirmed that there was no observable reaction between H2O2 and aniline at the reaction temperature. Then 1.0 g selected catalyst (or 5.0 gcat Lwater−1) was added to the reactor in order to initiate the catalytic oxidation reaction.
A 2.0 mL liquid was sampled from the reaction mixture at certain time intervals, and then centrifuged to separate solid residue. The liquid sample was then analyzed by a HACH DR 1010 chemical oxygen demand (COD) analyzer or a TOC-LCPH/CPN total organic carbon content (TOC) analyzer. The catalytic performance of a certain catalyst was evaluated by calculating the COD or TOC removal rate of the reaction mixture where the catalyst was used.
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