The materials included SY [Shanxi Huahui Kaide Pharmaceutical Co., Ltd. (Beijing, China), batch No. 20120725 ] and kits for determining total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (
LDL-C) (Wako Pure Chemical Industries, Ltd., Osaka, Japan). Simvastatin (SIM; Henan Dikang Pharmaceutical Co., Ltd., Henan, China) was prepared as a 15 mg/mL stock solution in dimethyl sulfoxide and stored at -8°C. Amphotericin B (North China Pharmaceutical Co., Ltd., Shanghai, China) was prepared as a 30 mg/mL stock solution in dimethyl sulfoxide, stored at -8°C, and used within 3 days.
Lipoprotein-deficient serum was obtained from Biomedical Technologies, Inc., Stoughton, MA, USA. Mevalonic lactone was from Merck (White House Station, NJ, USA). The Chinese hamster ovary (CHO) cell line was from the Experimental Animal Center (Beijing University). The TC2323 CO 2 incubator was from LEAD-TECH (Shanghai) Scientific Instrument Co., Ltd., Shanghai, China. The CH2-TKC-3 inverted optical microscope was from Darkoo Optics Co., Ltd., Guangdong, China. The DG-5031 enzyme-linked immunosorbent assay analyzer was from Midwest Taian Technology Service Co., Ltd. (Beijing, China).
Bao L.D., Wang Y., Ren X.H., Ma R.L., Lv H.J, & Agula B. (2015). Hypolipidemic effect of safflower yellow and primary mechanism analysis. Genetics and molecular research : GMR, 14(2).