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Ldl c

Manufactured by Fujifilm
Sourced in Japan

The LDL-C is a laboratory instrument used to measure the concentration of low-density lipoprotein cholesterol (LDL-C) in blood samples. It provides accurate and reliable results to assist healthcare professionals in assessing an individual's cardiovascular health and risk of heart disease.

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2 protocols using ldl c

1

Comprehensive Biomarker Analysis in Plasma

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The concentrations of CEA and CYFRA211 in the plasma were assessed using electrochemiluminescence immunoassay (ECLIA) kits (Roche, Germany) on a Roche E170 fully automatic electrochemistry luminescence immunity analyzer (Roche, German). The levels of SCCA in the plasma were detected using an ARCHITECT I2000SR immune analyze system (Abbott, America). Each test included a standard control (CV < 5%).
The plasma biochemical indices including TG, CHO, HDL-C, LDL-C(WAKO, Japan), ApoA1, Apo-B(Maccura,China) and inflammatory biomarker CRP (WAKO, Japan) were measured on an automatic biochemical analyzer (LABOSPECT 008; Hitachi HighTechnologies Corporation, Japan).
The plasma hemostasis indices including PT, APTT, FBG, TT, DD (Siemens, Germany) and FDP (BIOLINKS CO., LTD, Japan) were measured on an automatic coagulation analyzer (SYSMEX CS-5100 Hemostasis System, Japan). PT% and INR were calculated according to the formula recommended by the manufacture’s instruction.
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2

Cholesterol Metabolism in CHO Cells

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The materials included SY [Shanxi Huahui Kaide Pharmaceutical Co., Ltd. (Beijing, China), batch No. 20120725 ] and kits for determining total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) (Wako Pure Chemical Industries, Ltd., Osaka, Japan). Simvastatin (SIM; Henan Dikang Pharmaceutical Co., Ltd., Henan, China) was prepared as a 15 mg/mL stock solution in dimethyl sulfoxide and stored at -8°C. Amphotericin B (North China Pharmaceutical Co., Ltd., Shanghai, China) was prepared as a 30 mg/mL stock solution in dimethyl sulfoxide, stored at -8°C, and used within 3 days. Lipoprotein-deficient serum was obtained from Biomedical Technologies, Inc., Stoughton, MA, USA. Mevalonic lactone was from Merck (White House Station, NJ, USA). The Chinese hamster ovary (CHO) cell line was from the Experimental Animal Center (Beijing University). The TC2323 CO 2 incubator was from LEAD-TECH (Shanghai) Scientific Instrument Co., Ltd., Shanghai, China. The CH2-TKC-3 inverted optical microscope was from Darkoo Optics Co., Ltd., Guangdong, China. The DG-5031 enzyme-linked immunosorbent assay analyzer was from Midwest Taian Technology Service Co., Ltd. (Beijing, China).
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