Luteolin

Manufactured by Tokyo Chemical Industry

Sourced in Japan

Luteolin is a flavonoid compound that serves as a standard reference material for analytical and research purposes. Its core function is to act as a chemical marker for quality control and identification in various applications.

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Lab products found in correlation

Quercetin, by Merck Group (1 mentions) Span 80, by Tokyo Chemical Industry (1 mentions) Nerolidol, by Thermo Fisher Scientific (1 mentions) Phosal 50 pg, by Lipoid (1 mentions) Tween 80, by Tokyo Chemical Industry (1 mentions) 1 8 cineole, by Thermo Fisher Scientific (1 mentions) Tween 20, by Merck Group (1 mentions) Kolliphor el, by BASF (1 mentions) Non essential amino acids neaa, by Merck Group (1 mentions) Fisetin, by Thermo Fisher Scientific (1 mentions) Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions) Sodium phosphate monobasic, by Merck Group (1 mentions) Resomer rg 502h, by Evonik (1 mentions) Quercetin, by Tokyo Chemical Industry (1 mentions) Apigenin, by Tokyo Chemical Industry (1 mentions) Mevalonate, by Merck Group (1 mentions) Fluvastatin, by Merck Group (1 mentions) Lipoprotein deficient serum, by Merck Group (1 mentions) Dii labeled ldl, by Thermo Fisher Scientific (1 mentions) U0126, by Merck Group (1 mentions)

5 protocols using luteolin

Methanol Extraction of Larix Kaempferi Sawdust

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LK-ME used in this study was prepared by methanol extraction from saw dust of L. kaempferi. Saw dust of L. kaempferi was obtained from the Forestry cooperative of Shimokawa town, Hokkaido, Japan. The saw dust (7.43 g) was extracted with 40 ml of methanol overnight at room temperature. The debris was removed by centrifugation, and the supernatant was filtrated with a 0.45 μm filter. Then, 1 ml of the extract was dried, resolved into 150 μl of dimethyl sulfoxide (DMSO, for assays using cultured cells) or ethanol (for glycation assay), and used in this study.
Purified taxifolin (AdooQ Bioscience, Irvine, CA, USA), quercetin (Sigma-Aldrich, St. Louis, MO, USA), and luteolin (Tokyo Chemical Industry, Tokyo, Japan) were purchased from commercially available products.

Muramatsu D., Uchiyama H., Kida H, & Iwai A. (2019). Cell cytotoxity and anti-glycation activity of taxifolin-rich extract from Japanese larch, Larix kaempferi. Heliyon, 5(7), e02047.

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Luteolin-Enriched Topical Formulation

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Luteolin [2-(3,4-dihydroxyphenyl)-5,7-dihydroxychromen-4-one, CAS#491-70-3)] with a purity >98%, Tween^® 80 (Polyoxyethylene 20 sorbitan monooleate, CAS#9005-65-6), and Span^® 80 [(Z)-Sorbitan mono-9-octadecenoate, CAS#1338-43-8] were purchased from Tokyo Chemical Industry (Tokyo, Japan). Castor oil, jojoba oil, and shea butter were obtained from Magie Fairy (Taichung city, Taiwan). PHOSAL^® 50 PG (Phosphatidylcholine in propylene glycol, content ≥ 50.0%) was obtained from LIPOID^® GmbH (Ludwigshafen, Germany). Kolliphor^® EL (Polyethoxylated castor oil, CAS#61791-12-6) was supplied by BASF (Ludwigshafen, Germany) and Tween^® 20 (Polyoxyethylene 20 sorbitan monolaurate, CAS#9005-64-5) was from Sigma-Aldrich Co. (St. Louis, MO, USA). 1,8-cineole (1,3,3-trimethyl-2-oxabicyclo [2.2.2]octane, CAS# 470-82-6) and nerolidol [(6E)-3,7,11-trimethyldodeca-1,6,10-trien-3-ol, CAS# 7212-44-4)] were purchased from Alfa Aesar (Haverhill, MA, USA). The MultiScreen^® Permeability Filter Plate, 0.4 µm, non-sterile (The skin PAMPA kit) was from Merck (Darmstadt, Germany). All solvents used in this study were of analytical grade.

Hsieh Y.S., Chen Y.F., Cheng Y.Y., Liu W.Y, & Wu Y.T. (2022). Self-Emulsifying Phospholipid Preconcentrates for the Enhanced Photoprotection of Luteolin. Pharmaceutics, 14(9), 1896.

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Luteolin Cytotoxicity Evaluation

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Luteolin was purchased from Tokyo Chemical Industry (Tokyo, Japan). Dimethyl sulfoxide (DMSO), phorbol 12-myristate 13-acetate (PMA), all-trans-retinoic acid (ATRA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), propidium iodide (PI), RPMI-1640 medium, nonessential amino acids (NEAA) and other chemicals were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA) unless otherwise indicated.

Chen P.Y., Tien H.J., Chen S.F., Horng C.T., Tang H.L., Jung H.L., Wu M.J, & Yen J.H. (2018). Response of Myeloid Leukemia Cells to Luteolin is Modulated by Differentially Expressed Pituitary Tumor-Transforming Gene 1 (PTTG1) Oncoprotein. International Journal of Molecular Sciences, 19(4), 1173.

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Fisetin and Luteolin Encapsulation in PLGA

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Fisetin (purity 96%) was purchased from Alfa Aesar (Haverhill, MA, USA). Luteolin (purity 98%) was from Tokyo Chemical Industries (Tokyo, Japan). PLGA (50:50, Mw 7000–17,000, Resomer^® RG 502H) was purchased from Evonik Industries AG (Essen, Germany). Poly (vinyl alcohol) (Mw 9000–10,000) and sodium phosphate monobasic were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Acetone was purchased from Echo Chemical Co. (Miaoli County, Taiwan). HPLC grade acetonitrile was obtained from Fisher Scientific (Hampton, NJ, USA).

Liu W.Y., Lin C.C., Hsieh Y.S, & Wu Y.T. (2021). Nanoformulation Development to Improve the Biopharmaceutical Properties of Fisetin Using Design of Experiment Approach. Molecules, 26(10), 3031.

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Regulation of Lipoprotein Metabolism

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Kaempferol, fluvastatin, mevalonate, lipoprotein-deficient serum (LPDS), and U0126 were purchased from Sigma. Quercetin, apigenin, and luteolin were purchased from Tokyo chemical industry. Kaempferol, Quercetin, apigenin, luteolin, and U0126 were dissolved in dimethylsulfoxide (DMSO), and Kaempferol was prepared at the time of use. In this study, the final DMSO concentration in the cultured medium was 0.1%. DiI-labeled LDL was obtained from Molecular Probes.

Ochiai A., Miyata S., Iwase M., Shimizu M., Inoue J, & Sato R. (2016). Kaempferol stimulates gene expression of low-density lipoprotein receptor through activation of Sp1 in cultured hepatocytes. Scientific Reports, 6, 24940.

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