α amylase

Manufactured by HiMedia

Sourced in India, United States

α-amylase is an enzyme that catalyzes the hydrolysis of α-1,4-glycosidic linkages in starch, glycogen, and related polysaccharides. It is a widely used enzyme in various industries, including food, textile, and pharmaceutical.

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Lab products found in correlation

7 protocols using α amylase

Antioxidant and Enzyme Inhibition Assays

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1,1–Diphenyl–2–picrylhydrazyl (DPPH), gallic acid and quercetin were purchased from Wako Pure Chemicals, Osaka, Japan. α-Amylase was obtained from Hi-Media Laboratories, Mumbai, India. Ascorbic acid, aluminium chloride and starch were procured from Qualigens Fine Chemicals, Mumbai, India. Folin-Ciocalteu’s phenol reagent was obtained from Sigma Aldrich, St. Louis, MO, USA.

Sai K., Thapa R., Devkota H.P, & Joshi K.R. (2019). Phytochemical Screening, Free Radical Scavenging and α-Amylase Inhibitory Activities of Selected Medicinal Plants from Western Nepal. Medicines, 6(2), 70.

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Chromatographic Analysis of Antioxidant Compounds

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Chloroform, methanol (Calro Ebra reagents, France), type G silica gel F₂₅₄ (Sigma-Aldrich, USA), 3,5-dinitro salicylic acid (DNSA) (Sigma-Aldrich, USA), 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) (Sigma-Aldrich, USA) and α-amylase (HiMedia, Mumbai, India) were used to conduct this study, and all were analytical grade and procured from the market.
20 20 cm glass plate, thin-layer chromatography (TLC) jar (CAMAG, Germany), automatic TLC coating machine (CAMAG, Germany), ultraviolet (UV) lamp (CAMAG, Germany), double beam ultraviolet-visible (UV-VIS) spectrophotometer (P.G. instrument ltd., China), test tubes, micropipette and analytical weighing balance (adventurer OHAUS, China) were the equipments employed to perform this investigation

Tekulu G.H., Araya E.M, & Mengesha H.G. (2019). In vitro α-amylase inhibitory effect of TLC isolates of Aloe megalacantha baker and Aloe monticola Reynolds. BMC Complementary and Alternative Medicine, 19, 206.

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Protein Interaction Protocols

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4-Chloro-7-nitrobenzofurazan, warfarin, trypsin (from bovine pancreas), α-Chymotrypsin (from bovine pancreas) and α-lactalbumin (from bovine milk) were purchased from Sigma-Aldrich (India). n-Butyl amine was purchased from Spectrochem (India). HSA, RNase A (from bovine pancreas, free from DNase), Catalase (from the bovine liver), α-Amylase and DNase (from bovine pancreas) were purchased from HIMEDIA (USA). BSA was purchased from BIOMATIK, USA. All the solvents were of spectroscopic grade and were used without any further purification. Milli-Q grade water was used for the spectroscopic measurements with a resistivity of 18.2 MΩ·cm at 298 K.

Dutta T., Pal K, & Koner A.L. (2020). Cellular metabolic activity marker via selective turn-ON detection of transporter protein using nitrobenzoxadiazole-based fluorescent reporter. Scientific Reports, 10, 4166.

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Enzymatic Carbohydrate Metabolism Protocol

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The dialysis membrane, 1,4-α-D glucan-glucanohydrolase, α-amylase, α-glucosidase, P-nitrophenyl-α-D-glucopyranoside, and acarbose were purchased from Himedia Laboratories, Mumbai, India. All other chemicals and reagents were AR grade purchased locally.

Bharathi A., Roopan S.M., Vasavi C.S., Munusami P., Gayathri G.A, & Gayathri M. (2014). In Silico Molecular Docking and In Vitro Antidiabetic Studies of Dihydropyrimido[4,5-a]acridin-2-amines. BioMed Research International, 2014, 971569.

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Bioconversion of Rice Straw to Biofuels

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The RS was collected from a nearby field in Imphal East District, Manipur, India. The RS was dried, chipped, and ground to size particles less than 1 mm. The finely powdered RS was thoroughly washed with double distilled water (ddw) to eliminate all the soluble contents present in it. The rinsing with distilled water continued until the wash water became clear. The commercial cellulase enzyme (ONOZUKA R-10) and α-amylase (from Malt) were obtained from HIMEDIA, Mumbai, India; amyloglucosidase obtained from Aspergillus niger was procured from SIGMA, United States. Furthermore, the cellulolytic fungus Aspergillus flavus (Accession No. OK330386) was isolated from a soil sample to check its cellulase activity. Saccharomyces cerevisiae (Accession No. OK189580), which is ethanologenic, was isolated from grapes to check its fermentation efficiency.

Ningthoujam R., Jangid P., Yadav V.K., Sahoo D.K., Patel A, & Dhingra H.K. (2023). Bioethanol production from alkali-pretreated rice straw: effects on fermentation yield, structural characterization, and ethanol analysis. Frontiers in Bioengineering and Biotechnology, 11, 1243856.

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Cassava Starch Extraction Using Malt α-Amylase

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Cassava root was purchased from a farmer market from Chera Dynasty, Kerala. The enzymes α-Amylase extracted from malt obtained procured from Hi-Media Lab, Mumbai. Enzyme activity was 1:2000 I.P. Units. All the chemicals used in this investigation were of analytical grade

Dolas K., Ranveer R.C., Tapre A.R., Nandane A.S., & Sahoo A.K. (2020). Effect of starch modification on physico-chemical, functional and structural characterization of cassava starch (Manihot esculenta Crantz). Food Research, 4(4), 1265-1271.

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Antioxidant and Antidiabetic Assays

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Folin-Ciocalteu’s reagent, sodium carbonate, aluminium chloride, potassium acetate, quercetin, Mueller-Hilton agar medium, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), potassium persulfate, ammonium molybdate, trisodium phosphate, potassium ferrocyanide, glucose, hemoglobin, sodium azide, 3, 5-dinitrosalicylic acid, ascorbic acid and α-amylase were purchased from Himedia (Bangalore, India). α- glucosidase, p-NPG (p-nitro phenyl α-d-glucopyranoside)), gallic acid, acarbose was purchased from Sigma–Aldrich (Bangalore, India). Standard anti-microbial drug, gentamycin and antidiabetic drug, metformin were purchased from the local market. All other chemical reagents and buffers were used as an analytical grade in this study.

Yarrappagaari S., Gutha R., Narayanaswamy L., Thopireddy L., Benne L., Mohiyuddin S.S., Vijayakumar V, & Saddala R.R. (2020). Eco-friendly synthesis of silver nanoparticles from the whole plant of Cleome viscosa and evaluation of their characterization, antibacterial, antioxidant and antidiabetic properties. Saudi Journal of Biological Sciences, 27(12), 3601-3614.

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