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Bond rxm research stainer

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The BOND RXm Research Stainer is an automated immunohistochemistry (IHC) and in-situ hybridization (ISH) staining system. It is designed for research applications, providing consistent and reproducible staining results.

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Lab products found in correlation

Diamond antifade mounting medium, by Thermo Fisher Scientific (4 mentions) Spectral dapi, by Akoya Biosciences (4 mentions) Opal fluorophore, by Akoya Biosciences (3 mentions) Opal polymer hrp secondary antibody, by Akoya Biosciences (3 mentions) Ae1 ae3, by Agilent Technologies (2 mentions) Ar9640, by Leica (1 mentions) Ccnd1, by Thermo Fisher Scientific (1 mentions) Vectra polaris automated quantitative pathology imaging system, by Akoya Biosciences (1 mentions) D5d5r, by Cell Signaling Technology (1 mentions) E1l3n, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Bond RXm (65 citations) Leica stainer (2 citations)

4 protocols using bond rxm research stainer

1

Multiplex Immunofluorescence for Cell Cycle Markers

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Formalin-fixed Paraffin-embedded (FFPE) tissue sections orTMAs were cut at 4–5 µm on charged slides. Slides were dried at 65°C for 6 hours. After drying, the slides were placed on the BOND RXm Research Stainer (Leica Biosystems) and deparaffinized with BOND Dewax solution (AR9222, Lecia Biosystems). The multispectral immunofluorescent (mIF) staining process involved serial applications of the following for each biomarker: epitope retrieval/stripping with ER1 (citrate buffer pH 6, AR996, Leica Biosystems ), blocking buffer (AKOYA Biosciences), primary antibody, Opal Polymer HRP secondary antibody (AKOYA Biosciences), Opal Fluorophore (AKOYA Biosciences). All AKOYA reagents used for mIF staining come as a kit (NEL821001KT). Spectral DAPI (AKOYA Biosciences) was applied once slides were removed from the BOND. They were cover slipped using an aqueous method and Diamond antifade mounting medium (Invitrogen ThermoFisher). The mIF panel consisted of the following antibodies (clone, company, and Opal Fluorophores): Cyclin D (SP4, Epredia, Opal 570), Cyclin E (EP435E, abcam, Opal 520), MCM2 (RBT-MCM2, Biosb, Opal Polaris 480), Pan Cytokeratin (AE1AE3, Agilent DAKO, Opal Polaris 780), pHH3 (Ser10, Millipore Sigma, Opal 620), pRB (Ser807/811, Cell Signaling, Opal 690).
Knudsen E.S., Kumarasamy V., Nambiar R., Pearson J.D., Vail P., Rosenheck H., Wang J., Eng K., Bremner R., Schramek D., Rubin S.M., Welm A.L, & Witkiewicz A.K. (2022). CDK/cyclin dependencies define extreme cancer cell-cycle heterogeneity and collateral vulnerabilities. Cell reports, 38(9), 110448.
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2

Multiplex Immunofluorescence Staining of FFPE Sections

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Formalin-fixed paraffin-embedded (FFPE) 4 µm sections were cut and placed on charged slides. Slides were dried at 65 °C for 2 h. After drying, the slides were placed on the BOND RXm Research Stainer (Leica Biosystems) and deparaffinized with BOND Dewax solution (AR9222, Lecia Biosystems). The multispectral immunofluorescent (mIF) staining process involved serial repetitions of the following for each biomarker: epitope retrieval/stripping with ER1 (citrate buffer pH 6, AR996, Leica Biosystems) or ER2 (Tris-EDTA buffer pH9, AR9640, Leica Biosystems), blocking buffer (AKOYA Biosciences), primary antibody, Opal Polymer HRP secondary antibody (AKOYA Biosciences), Opal Fluorophore (AKOYA Biosciences). All AKOYA reagents used for mIF staining come as a kit (NEL821001KT). Spectral DAPI (AKOYA Biosciences) was applied once slides were removed from the BOND. They were cover slipped using an aqueous method and Diamond antifade mounting medium (Invitrogen ThermoFisher). The mIF panel consisted of the following antibodies: Ki67 (Abcam, ab16667), AE1AE3 (Dako, M3515), CCNE (Abcam, ab33911), CCND1 (ThermoFisher, MA1-39546), CCNA (Abcam, ab32386), RB (Cell Signaling, 9309s), pRB (Cell Signaling, 8516), and MCM2 (BioSb, BSB6334).
Witkiewicz A.K., Schultz E., Wang J., Hamilton D., Levine E., O’Connor T, & Knudsen E.S. (2023). Determinants of response to CDK4/6 inhibitors in the real-world setting. NPJ Precision Oncology, 7, 90.
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3

Multiplex Immunofluorescence for Cell Cycle Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin-fixed Paraffin-embedded (FFPE) tissue sections orTMAs were cut at 4–5 µm on charged slides. Slides were dried at 65°C for 6 hours. After drying, the slides were placed on the BOND RXm Research Stainer (Leica Biosystems) and deparaffinized with BOND Dewax solution (AR9222, Lecia Biosystems). The multispectral immunofluorescent (mIF) staining process involved serial applications of the following for each biomarker: epitope retrieval/stripping with ER1 (citrate buffer pH 6, AR996, Leica Biosystems ), blocking buffer (AKOYA Biosciences), primary antibody, Opal Polymer HRP secondary antibody (AKOYA Biosciences), Opal Fluorophore (AKOYA Biosciences). All AKOYA reagents used for mIF staining come as a kit (NEL821001KT). Spectral DAPI (AKOYA Biosciences) was applied once slides were removed from the BOND. They were cover slipped using an aqueous method and Diamond antifade mounting medium (Invitrogen ThermoFisher). The mIF panel consisted of the following antibodies (clone, company, and Opal Fluorophores): Cyclin D (SP4, Epredia, Opal 570), Cyclin E (EP435E, abcam, Opal 520), MCM2 (RBT-MCM2, Biosb, Opal Polaris 480), Pan Cytokeratin (AE1AE3, Agilent DAKO, Opal Polaris 780), pHH3 (Ser10, Millipore Sigma, Opal 620), pRB (Ser807/811, Cell Signaling, Opal 690).
Knudsen E.S., Kumarasamy V., Nambiar R., Pearson J.D., Vail P., Rosenheck H., Wang J., Eng K., Bremner R., Schramek D., Rubin S.M., Welm A.L, & Witkiewicz A.K. (2022). CDK/cyclin dependencies define extreme cancer cell-cycle heterogeneity and collateral vulnerabilities. Cell reports, 38(9), 110448.
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4

Multiplexed Immunofluorescence Staining of FFPE Tumor Samples

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Tumor samples were fixed in formalin and embedded in paraffin (FFPE) and 4 μm thick sections were cut for staining. A pathologist (AW) evaluated hematoxylin and eosin (H&E) stained slides from each FFPE block. MIF staining was performed on the BOND RXm Research Stainer (Leica Biosystems). The MIF panel consisted of the following antibodies (clone, company, and opal fluorophores): TIM3 (D5D5R™, Cell Signaling, Opal 540), CTLA4 (UMAB249, Biocare Medical, Opal 570), OX40 (E9U7O, Cell Signaling, Opal 520), PD-L1 (E1L3N, Cell Signaling, Opal 620), LAG3 (D2G4O, Cell Signaling, Opal 650), pancytokeratin (AE1AE3, Agilent DAKO, Opal 690). All AKOYA reagents used for MIF staining are part of a detection kit (NEL821001KT). Spectral DAPI (AKOYA Biosciences) was applied once slides were removed from the BOND. They were cover slipped using an aqueous method and Diamond antifade mounting medium (Invitrogen, ThermoFisher). Slides were imaged on the Vectra® Polaris Automated Quantitative Pathology Imaging System (AKOYA Biosciences). Spectral unmixing, tissue and cell segmentation, and cell phenotyping were performed on inForm® Software v2.4.8 (AKOYA Biosciences).
Hellström I., Ledbetter J.A., Scholler N., Yang Y., Ye Z., Goodman G., Pullman J., Hayden-Ledbetter M, & Hellström K.E. (2001). CD3-mediated activation of tumor-reactive lymphocytes from patients with advanced cancer. Proceedings of the National Academy of Sciences of the United States of America, 98(12), 6783-6788.
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