The fatty acid composition of the murine liver and serum samples was analyzed using gas chromatography-mass spectrometry on an Agilent 7890B/5977B System (Agilent Technologies, Santa Clara, CA, USA). Liver tissue (15 µg) and serum (25 µL) samples were methylated using a Fatty Acid Methylation Kit (Nacalai Tesque, Kyoto, Japan). The final product was loaded onto a Varian capillary column (DB-FATWAX UI; Agilent Technologies). The capillary column used for fatty acid separation was CP-Sil 88 for FAME (100 m × an inner diameter of 0.25 mm × membrane thickness of 0.20 μm, Agilent Technologies). The column temperature was maintained at 100°C for 4 min, increased gradually by 3°C/min to 240°C, and then held there for 7 min. The sample was injected in split mode with a split ratio of 5:1. Each fatty acid methyl ester was detected in the select ion-monitoring mode. All results were normalized to the peak height of the C17:0 internal standard (14 (link)).
Agilent 7890b 5977b system
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 7890B/5977B System is a gas chromatograph-mass spectrometer (GC-MS) instrument. It is designed for the analysis of complex mixtures of volatile and semi-volatile organic compounds. The system combines the Agilent 7890B gas chromatograph and the Agilent 5977B mass spectrometer to provide high-performance separation and detection capabilities.
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Lab products found in correlation
2 protocols using agilent 7890b 5977b system
1
Murine Liver and Serum Fatty Acids
2
GC-MS Metabolite Profiling and Analysis
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The samples were acted on by an Agilent 7890B-5977B system (Agilent, Palo Alto, CA, USA). A 30 m × 0.25 mm × 0.25 µm HP-5MS fused-silica capillary column (Agilent J & W Scientific, Folsom, CA, USA) was used to separate the derivatives. Helium (>99.999%) was used as the carrier gas at a rate of 1 mL/min, and the injector temperature was maintained at 260 °C. The temperature of the mass spectrometry (MS) quadrupole was set to 150 °C, the ion source was 230 °C, and the collision energy was 70 eV. Mass spectrometric data were obtained in full-scan mode (m/z 50-500). The samples were individually run, and quality control was performed. Partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to visualize metabolic differences between the two groups using the SIMCA version 14.1 software (Umetrics, Umeå, Sweden). Metabolites were screened using the National Institute of Standards and Technology database (NIST). Enrichment of metabolic pathway was based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database.
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