Anti beclin 1 nb110 87318

Forskolin (F6886), NH₄Cl (A9434) and leupeptin (L2884) were purchased from Sigma–Aldrich (St. Louis, MO, USA). H89 (#371962) and KT5823 (#420321) were from Calbiochem (San Diego, CA, USA). Anti-perilipin 1 (#9349), anti-HSL (#4107), anti-pHSL (Ser552)(#4139), anti-pHSL (Ser554)(#4137), anti-pHSL (Ser650)(#4126), anti-acetyl-CoA carboxylase (#3676), anti-adiponectin (#2789), anti-CCAAT/enhancer-binding protein α (C/EBPα) (#8178), anti-fatty acid binding protein 4 (FABP4) (#3544) and anti-fatty acid synthase (FAS) (#3180), anti-p62/SQSTM1 (#5114s) antibodies were from Cell Signaling Technology (Beverly, MA, USA). Anti-beclin-1 (NB110-87318), anti-Atg5 (NB110-53818), and anti-LC3 antibody (NB100-2220) was purchased from Novus Biologicals (Littleton, CO, USA). Anti-GAPDH (SC-25778) antibody was from Santa Cruz Biotechnology (Dallas, TX, USA).

The selective CTSS inhibitor Z-FL-COCHO (ZFL) was purchased from Calbiochem Co. (Darmstadt, Germany). Temozolomide (TMZ, 76899) and BafilomycinA1 (BafA1) was purchased from Sigma-Aldrich (Missouri, USA). BAPTA-AM was purchased from Selleck Chemicals (Shanghai, China). Anti-Bax (#5023), anti-Bcl-2 (#3498), anti-cleaved caspase 3 (#9661), anti-PARP (#9532), anti-Atg3 (#3415), anti-Atg5 (#9980), anti-Atg7 (#2631), anti-Atg12 (#2010), and anti-β-actin (#4970) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-LC3II (NB100-2220) and anti-Beclin 1 (NB110-87318) antibodies were purchased from Novus Biologicals (Littleton, CO, USA). Anti-CTSS (ab134157), anti-CD31 (ab28364), anti-MCU (ab121499), anti-MICU1 (ab190114), anti-MICU2 (ab101465), anti-EMRE (ab122209), and anti-p62/SQSTM1 (ab91526) antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-Mff (17090-1-AP), anti-Drp1 (12957-1-AP), and anti-OPA1 (27733-1-AP) antibodies were purchased from Proteintech Inc(Rosemont, MA, USA).