Cells were seeded at 3 × 105 cells per well in a 48-well culture plate (Corning, New York, NY, USA) and grown in serum-free medium overnight. The next day, cells were pretreated with CA and RA alone or in combination with indicated concentrations for 8 h. The supernatants were collected and subjected to centrifugation at 10,000× g for 10 min at 4 °C. The secreted IL-1β in the supernatant was then quantified using an enzyme-linked immunosorbent assay (ELISA) kit for human IL-1β (Raybiotech, Norcross, GA, USA) following the manufacturer’s protocol [18 (link)]. Concentrations of measured IL-1β were normalized to the cell number determined in parallel.
Elisa kit for human il 1β
Manufactured by RayBiotech
Sourced in United States
The ELISA kit for human IL-1β is a quantitative sandwich enzyme-linked immunosorbent assay designed to measure the concentration of IL-1β in human samples. It utilizes a specific antibody coated on a microplate to capture the target analyte from the sample, which is then detected using a second, enzyme-labeled antibody. The resulting color change is proportional to the amount of IL-1β present in the sample.
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Lab products found in correlation
2 protocols using elisa kit for human il 1β
1
Quantifying Secreted IL-1β in Cells
2
Quantifying Secreted IL-1β in Cells
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Cells were seeded at 3 × 105 cells per well in a 48-well culture plate (Corning, New York, NY, USA) and grown in serum-free medium overnight. The next day, cells were pretreated with CA and RA alone or in combination with indicated concentrations for 8 h. The supernatants were collected and subjected to centrifugation at 10,000× g for 10 min at 4 °C. The secreted IL-1β in the supernatant was then quantified using an enzyme-linked immunosorbent assay (ELISA) kit for human IL-1β (Raybiotech, Norcross, GA, USA) following the manufacturer’s protocol [18 (link)]. Concentrations of measured IL-1β were normalized to the cell number determined in parallel.
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