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Ros glo bioluminescent assay

Manufactured by Promega
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The ROS-Glo bioluminescent assay is a laboratory equipment product that measures reactive oxygen species (ROS) levels in cell-based and cell-free systems. It utilizes a luminogenic substrate that reacts with ROS to generate a bioluminescent signal proportional to the ROS concentration.

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Lab products found in correlation

White 96 well plate, by Greiner (2 mentions)

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ROS-Glo assay (19 citations) ROS-Glo (10 citations)

2 protocols using ros glo bioluminescent assay

1

Quantifying Neutrophil Reactive Oxygen Species

Check if the same lab product or an alternative is used in the 5 most similar protocols
ROS were measured using the ROS-Glo bioluminescent assay (Promega) for the direct detection of H2O2. Briefly, neutrophils (2 × 104) were plated in white 96-well plates (Greiner) in high glucose DMEM medium. Cells were cultured with DMSO vehicle, 0.5 μM NA-11, 5 μM LDC7559, 100 μM apocynin, or 200 μM thionicotinamide for 30 min, and then stimulated with 50 nM PMA or treated with 10 μg/mL zymosan beads, pre-opsonized in 20% serum for 30 min. Luminescence, after the addition of H2O2 substrate solution of 20 min, and then ROS-Glo detection solution for 20 min, was recorded on a plate reader (Envision).
Amara N., Cooper M.P., Voronkova M.A., Webb B.A., Lynch E.M., Kollman J.M., Ma T., Yu K., Lai Z., Sangaraju D., Kayagaki N., Newton K., Bogyo M., Staben S.T, & Dixit V.M. (2021). Selective activation of PFKL suppresses the phagocytic oxidative burst. Cell, 184(17), 4480-4494.e15.
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2

Quantifying Neutrophil Reactive Oxygen Species

Check if the same lab product or an alternative is used in the 5 most similar protocols
ROS were measured using the ROS-Glo bioluminescent assay (Promega) for the direct detection of H2O2. Briefly, neutrophils (2 × 104) were plated in white 96-well plates (Greiner) in high glucose DMEM medium. Cells were cultured with DMSO vehicle, 0.5 μM NA-11, 5 μM LDC7559, 100 μM apocynin, or 200 μM thionicotinamide for 30 min, and then stimulated with 50 nM PMA or treated with 10 μg/mL zymosan beads, pre-opsonized in 20% serum for 30 min. Luminescence, after the addition of H2O2 substrate solution of 20 min, and then ROS-Glo detection solution for 20 min, was recorded on a plate reader (Envision).
Amara N., Cooper M.P., Voronkova M.A., Webb B.A., Lynch E.M., Kollman J.M., Ma T., Yu K., Lai Z., Sangaraju D., Kayagaki N., Newton K., Bogyo M., Staben S.T, & Dixit V.M. (2021). Selective activation of PFKL suppresses the phagocytic oxidative burst. Cell, 184(17), 4480-4494.e15.
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