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Annexin 5 fitc annexin 5 apoptosis detection kit 2

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Annexin V-FITC is a fluorescent conjugate of the Annexin V protein, which binds to phosphatidylserine exposed on the surface of apoptotic cells. This product is a component of the Annexin V Apoptosis Detection Kit II, which is used to detect and quantify apoptotic cells.

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Lab products found in correlation

Accuri c6 plus, by BD (1 mentions)

Close spelling variants of this product

FITC Annexin V Apoptosis Detection Kit II FITC Annexin V Apoptosis Detection Kit II with propidium iodide (AV/PI) BD Pharmingen FITC Annexin V Apoptosis Detection Kit II Annexin V-fluorescein isothiocyanate (FITC) Apoptosis Detection Kit II Annexin V-FITC/PI Apoptosis detection Kit II FITC Annexin V Apoptosis Detection Kit FITC Annexin Apoptosis Detection Kit II Annexin V Apoptosis Detection Kit II FITC Annexin V Apoptosis Detection Annexin V-FITC Detection Kit II

2 protocols using annexin 5 fitc annexin 5 apoptosis detection kit 2

1

Apoptosis Induction by ABT737 and Cisplatin

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A total of 5×105 cells were plated in 6-well plates and incubated overnight at 37°C. Following exposure to ABT737 and/or cisplatin at 37°C for 24 h, the cells were collected. The induction of apoptosis in these cells was determined using Annexin V-FITC (Annexin V Apoptosis Detection Kit II, BD Biosciences, San Diego, CA, USA). The cells were analyzed using a flow cytometer (BD Biosciences).
Xu Y., Gao W., Zhang Y., Wu S., Liu Y., Deng X., Xie L., Yang J., Yu H., Su J, & Sun L. (2018). ABT737 reverses cisplatin resistance by targeting glucose metabolism of human ovarian cancer cells. International Journal of Oncology, 53(3), 1055-1068.
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2

Annexin V-FITC and PI Apoptosis Assay

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Annexin V-FITC (Annexin V Apoptosis Detection Kit II, BD Biosciences, San Diego, CA, United States) was used to detect cell apoptosis and propidium iodide (PI) was used to detect cell cycle. Exponentially growing QBC939 cells were seeded in 6 well culture plates at a density of 2 × 105 cells/well. After exposure to different experimental conditions, cells were trypsinized and resuspended in 1640 medium with 10% FBS at a concentration of 1 × 106 cells/ml. Samples were examined using the BD Accuri™ C6 Plus personal flow cytometer (Becton, Dickinson and Company, Franklin Lakes, NJ, United States).
Qin H., Zheng G., Li Q, & Shen L. (2023). Metabolic reprogramming induced by DCA enhances cisplatin sensitivity through increasing mitochondrial oxidative stress in cholangiocarcinoma. Frontiers in Pharmacology, 14, 1128312.
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