Cd112
The CD112 is a recombinant human protein produced in HEK293 cells. It is a member of the VISTA protein family and functions as an immune checkpoint regulator.
Lab products found in correlation
3 protocols using cd112
Characterization of GIST and CIK cells
NK Exosome-Mediated Cytotoxicity and Apoptosis
For experiments with blocking antibodies to LFA-1-L and DNAM1-L, before incubation with NK exosomes, NALM-18 cells were incubated for 30 minutes at 37 °C with 10 µg/mL of blocking antibodies to CD54 (R&D Systems, Minneapolis, USA), CD155 (Genetex, Irvine, CA, USA), CD112 (R&D Systems, Minneapolis, MN, USA). For DNAM1 blocking experiments, NK exosomes were previously incubated with anti-DNAM1 blocking mAb for 30 minutes at 37 °C and then incubated with target cells for 24 h.
Analysis of caspase activation was performed by using Vybrant FAM Caspase-3 and -7Assay Kit (Thermo Scientific, Waltham, MA, USA) according to manufacturer recommendations.
Immunophenotyping of γδ T Cells
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