To analyze spontaneous γH2AX and 53BP1 focus formation in primary liver cancer cells, cells were fixed in 4% paraformaldehyde for 10 min on ice, permeabilized with 0.5% Triton 100 for 10 min on ice, and blocked with 1% bovine serum albumin for 30 min. Subsequently, cells were stained with primary antibodies mouse anti-γH2AX monoclonal antibody (Abcam) or rabbit anti-53BP1 polyclonal antibody (Abcam) overnight and then with secondary antibody for 2 h. Cell nuclei were stained by 4′,6-diamidino-2-phenylindole (DAPI). γH2AX and 53BP1 foci were determined and counted under fluorescence microscope. Micronuclei were also stained with DAPI and counted. All experiments were repeated at least three times and images were captured by microscope.
Rabbit anti 53bp1 polyclonal antibody
Manufactured by Abcam
Rabbit anti-53BP1 polyclonal antibody is a primary antibody that recognizes the 53BP1 protein. 53BP1 is a DNA damage response protein that is involved in the detection and repair of DNA double-strand breaks. The antibody can be used to detect and study the expression and localization of 53BP1 in various cell types and experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rabbit anti 53bp1 polyclonal antibody
1
Quantifying DNA Damage Markers in Liver Cancer
2
Analyzing DNA Damage in Liver Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To analyze spontaneous γH2AX and 53BP1 focus formation in primary liver cancer cells, cells were xed in 4% paraformaldehyde for 10 min on ice, permeabilized with 0.5% Triton 100 for 10 min on ice, and blocked with 1% bovine serum albumin for 30 min. Subsequently, cells were stained with primary antibodies mouse anti-γH2AX monoclonal antibody (Abcam) or rabbit anti-53BP1 polyclonal antibody (Abcam) overnight and then with secondary antibody for 2 h. Cell nuclei were stained by 4',6-diamidino-2phenylindole (DAPI). γH2AX and 53BP1 foci were determined and counted under uorescence microscope. Micronuclei were also stained with DAPI and counted. All experiments were repeated at least three times and images were captured by microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!