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Phosphorylated mdm2

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Phosphorylated MDM2 is a recombinant protein that represents the phosphorylated form of the Murine Double Minute 2 (MDM2) protein. MDM2 is a key negative regulator of the tumor suppressor protein p53. Phosphorylation of MDM2 can modulate its function and interactions with other cellular proteins.

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Lab products found in correlation

Phosphorylated jak2 pjak2, by Cell Signaling Technology (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Phosphorylated stat3 p stat3, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Aurka, by Cell Signaling Technology (1 mentions) Phosphorylated erk1 2, by Cell Signaling Technology (1 mentions) Ki67 antibody, by Vector Laboratories (1 mentions) Phosphorylated β catenin, by Cell Signaling Technology (1 mentions) Lonafarnib, by Cayman Chemical (1 mentions) Cki 7, by Merck Group (1 mentions) Alamar blue, by Thermo Fisher Scientific (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Phosphorylated rb, by Cell Signaling Technology (1 mentions) Pd0325901, by Selleck Chemicals (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) Luciferin, by Promega (1 mentions) Ripa buffer, by Merck Group (1 mentions) Protease inhibitor, by Roche (1 mentions)

3 protocols using phosphorylated mdm2

1

Investigating EGFR and JAK2 Signaling

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RPMI-1640 medium, 0.05% trypsin–EDTA, and penicillin–streptomycin solution was obtained from Chemical Industry Co., Ltd. (Portland, OR, USA). Fetal bovine serum (FBS) and PFT-α (P4359) were also obtained from Sigma-Aldrich (Merck KGaA, St. Louis, MO, USA). Antibodies used for Western blotting, specifically phosphorylated EGFR (pEGFR; #3777), EGFR (#4267), phosphorylated JAK2 (pJAK2; (#3776), JAK2 (#3230), phosphorylated STAT3 (pSTAT3; #9145), STAT3 (#9139), p53 (#9282), phosphorylated MDM2 (#3521), MDM2 (#86934), and GAPDH (#2118) were bought from Cell Signaling Technology (Beverly, MA, USA). The antibody specific for PD-L1 (R30949) was obtained from NSJ Bioreagents (San Diego, CA, USA).
Sp N., Kang D.Y., Lee J.M, & Jang K.J. (2021). Mechanistic Insights of Anti-Immune Evasion by Nobiletin through Regulating miR-197/STAT3/PD-L1 Signaling in Non-Small Cell Lung Cancer (NSCLC) Cells. International Journal of Molecular Sciences, 22(18), 9843.
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2

Antibody Panel for Cell Signaling Analysis

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Rabbit polyclonal antibodies to GFP, phosphorylated ERK1/2, phosphorylated RB, ERK1/2, cyclin D1, phosphorylated FADD at Ser194, AURKA, phosphorylated β-Catenin, β-Catenin, phosphorylated MDM2, and PLK1 were purchased from Cell Signaling Technology. The antibody for human FADD was obtained from BD Biosciences. Antibodies against cyclin B1, CK1α, MDM2 and p53 were purchased from Santa Cruz Biotechnology. The antibody against mouse FADD used for western blotting was initially purchased from Epitomics (cat# 3523-1), and then from Abcam (cat# ab124812). Antibodies for BUB1, β-Actin, mouse FADD (cat# ab24533) and GFP were from Abcam. Ki-67 antibody was obtained from Vector Labs. CKI-7 was from Sigma. PD0325901 was purchased from Selleck, and Lonafarnib was bought from Cayman Chemical. Alamar Blue was purchased from Invitrogen. Luciferin was obtained from Promega. Adenovirus-Cre was bought from the University of Michigan Vector core.
Bowman B.M., Sebolt K.A., Hoff B.A., Boes J.L., Daniels D.L., Heist K.A., Galbán C.J., Patel R.M., Zhang J., Beer D.G., Ross B.D., Rehemtulla A, & Galbán S. (2015). Phosphorylation of FADD by the kinase CK1α promotes KRASG12D-induced lung cancer. Science signaling, 8(361), ra9.
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3

Western Blot Analysis of Kidney Protein

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We extracted protein from kidney tissues with the use of RIPA buffer (Sigma-Aldrich, Hamburg, Germany) that contained protease inhibitors (Roche) and processed it for Western blot analysis as described. 7 The membranes were incubated overnight at 4 C with primary antibodies, goat antibody against mouse p53 (Santa Cruz Biotechnology), rabbit antibody against MDM-2 (Abcam), phosphorylated-53 (Santa Cruz Biotechnology), phosphorylated-MDM2 (Cell Signaling Technology Inc., Danvers, MA), and rabbit antibody against b-actin (Cell Signaling Technology Inc.). Secondary antibodies were a peroxidase-conjugated donkey anti-goat IgG (Dianova) and an anti-rabbit IgG (Cell Signaling Technology Inc.).
Mulay S.R., Romoli S., Desai J., Honarpisheh M.M., Kumar S.V., Anders H.J, & Thomasova D. (2016). Murine Double Minute-2 Inhibition Ameliorates Established Crescentic Glomerulonephritis. The American journal of pathology, 186(6).
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