Anti nfat5 antibody

Antibodies were obtained as follows: anti-NFAT5 antibody was from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti-actin antibody was from Sigma (Deisenhofen, Germany); anti-phospho-GSK-3β (Ser9), anti-GSK-3β, anti-phospho-p38 (Thr180/Tyr182), and anti-p38 were purchased from Cell Signaling (Beverly, MA, USA); anti-phospho-Akt (Ser473) and anti-AKT were from Genscript (Piscataway, NJ, USA). GSK-3β inhibitor VIII was obtained from Cayman Chemical (Ann Arbor, MI, USA). Unless otherwise indicated, other reagents were purchased from Biomol (Hamburg, Germany), Biozol (Eching, Germany), Carl Roth (Karlsruhe, Germany), or Sigma.

Pharmacological inhibitors SB202190, SP600125, U0126, SrcI-1, and PF-228 were obtained from Sigma (Deisenhofen, Germany). Anti-NFAT5 antibody, anti-Src antibody, anti-FAK antibody, and anti-phospho-FAK antibody were from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti-actin antibody was from Sigma; anti-p38, anti-phospho-p38, anti-ERK1/2, anti-phospho-ERK1/2, anti-JNK, anti-phospho-JNK, and horseradish peroxidase-conjugated anti-rabbit IgG were purchased from Cell Signaling (Beverly, MA, USA); anti-phospho–Src antibody was from Abgent (Suzhou, China); anti-S100A4 antibody was from Spring Bioscience (Pleasanton, CA, USA). Accell SMARTpool siRNA constructs for knockdown of NFAT5 or S100A4, and Accell non-targeting siRNA (#2) were obtained from Thermo Fisher Scientific (Epsom, UK). Unless otherwise indicated, other reagents were purchased from Biomol (Hamburg, Germany), Biozol (Eching, Germany), Carl Roth (Karlsruhe, Germany), or Sigma.