Terminal deoxynucleotidetransferase

Manufactured by New England Biolabs

Terminal deoxynucleotidyl transferase (TdT) is an enzyme that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules in a template-independent manner. It plays a key role in the generation of antibody and T-cell receptor diversity during the process of V(D)J recombination in the immune system.

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Lab products found in correlation

Sep pak c18 cartridge, by Waters Corporation (2 mentions) α 32p cordycepin 5 triphosphate, by PerkinElmer (2 mentions) 394 dna synthesizer, by Thermo Fisher Scientific (2 mentions) α 32p dctp, by PerkinElmer (2 mentions) T4 polynucleotide kinase, by New England Biolabs (2 mentions) T4 dna ligase, by New England Biolabs (2 mentions) γ 32p atp, by PerkinElmer (2 mentions) Dna synthesis reagents, by Glen Research (1 mentions) α 32p dttp, by PerkinElmer (1 mentions) Klenow exo, by New England Biolabs (1 mentions)

2 protocols using terminal deoxynucleotidetransferase

Oligonucleotide Preparation and Analysis

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Oligonucleotides
were
prepared on an Applied Biosystems Inc. 394 DNA synthesizer. Commercially
available DNA synthesis reagents were obtained from Glen Research
Inc. DNA substrates used in this study are presented in Chart 1. T4 polynucleotide kinase, terminal deoxynucleotide
transferase, and T4 DNA ligase were obtained from New England Biolabs.
[α-³²P]dCTP, [γ-³²P]ATP, and [α-³²P]cordycepin 5′-triphosphate were purchased from PerkinElmer.
Analysis of radiolabeled oligonucleotides was conducted using a Storm
840 phosphorimager and ImageQuant TL. UvrABC was obtained as previously
described.^{36 (link),37 (link)} C18-Sep-Pak cartridges were obtained
from Waters. Experiments using radiolabeled oligonucleotides were
analyzed following PAGE using a Storm 840 phosphorimager and Imagequant
TL.

Ghosh S, & Greenberg M.M. (2014). Nucleotide Excision Repair of Chemically Stabilized Analogues of DNA Interstrand Cross-Links Produced from Oxidized Abasic Sites. Biochemistry, 53(37), 5958-5965.

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Oligonucleotide Synthesis and Radiolabeling

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Oligonucleotides were prepared on an Applied Biosystems Inc. 394 DNA synthesizer. Commercially available DNA synthesis reagents were obtained from Glen Research Inc. DNA substrates used in this study are presented in Figure 3. T4 polynucleotide kinase, Klenow exo^–, terminal deoxynucleotide transferase, and T4 DNA ligase were obtained from New England Biolabs. [α-³²P]dCTP, [α-³²P]-dTTP, [γ-³²P]ATP, and [α-³²P]cordycepin 5′-triphosphate were purchased from PerkinElmer. Analyses of radiolabeled oligonucleotides were conducted using a Storm 840 Phosphor-imager and ImageQuant TL software. C18-Sep-Pak cartridges were obtained from Waters.

Ghosh S, & Greenberg M.M. (2015). Correlation of Thermal Stability and Structural Distortion of DNA Interstrand Cross-Links Produced from Oxidized Abasic Sites with Their Selective Formation and Repair. Biochemistry, 54(40), 6274-6283.

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