Bio legend legend plex multiplex bead based assay

The NTA was performed using a nanoparticle tracking analyzer (NanoSight NS300; Malvern Instruments, Malvern, UK).
Transmission Electron Microscopy mADSC-Exos (5 μL) were added to formvar/carbon-coated copper grids, dried for 20 min, then briefly washed with ultrapure water, and stained with 1% uranyl acetate. The samples were visu-Flow Cytometry mADSCs (6 × 10 5 cells) were collected and washed in PBS containing 0.1% bovine serum albumin and 0.01% sodium azide; they were then incubated for 30 min at 4°C with FITC-conjugated anticluster of differentiation (CD) 44 (eBioscience, San Diego, CA, USA, 1:100), PE-conjugated anti-CD34 (Invitrogen, Waltham, MA, USA, 1:100), APC-conjugated anti-CD29 (eBioscience, 1:100), and PerCP-Cy5.5-conjugated anti-SCA-1 (eBioscience, 1:100) antibodies. After 3 washes in PBS and fixing with 0.1% formaldehyde, the cells were analyzed on a FACScan flow cytometer (Becton Dickinson, Franklin Lakes, NY, USA).
The cornea and conjunctiva were homogenized in lysis buffer and centrifuged at 12,000 g for 5 min at 4°C. The supernatant was then collected. Levels of pro-inflammatory cytokine were measured using BioLegend LEGENDplex TM multiplex bead-based assay from BioLegend (San Diego, CA, USA).